Abstract
The effects of some metal ions on amidolytic and fibrinogenolytic activities of highly purified human plasmin were investigated in vitro. In the presence of Zn2+, Cu2+, Cd2+, and Au+ in the incubation mixture at the concentrations of 1×10−5−1×10−3 M, the anidolytic plasmin activity was strongly inhibited, whereas Ca2+ and Mg2+ at the same concentrations were not effective. The analysis of the kinetic study has shown that Zn2+ or Cu2+ acts as mixed-type inhibitors of plasmin activity. The inhibition of amidolytic plasmin activity by Zn2+ and Cu2+ was reduced in the presence of EDTA, histidine, or albumin. Incubation of plasmin with Zn2+ or Cu2+ (at the concentration of 5×10−4 M) resulted in complete loss of its proteolytic action on fibrinogen, whereas Cd2+ and Au+ under the same conditions only partially inhibited this process.
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Nowak, P., Zgirski, A. Effects of metal ions on activity of plasmin. Biol Trace Elem Res 93, 87–94 (2003). https://doi.org/10.1385/BTER:93:1-3:87
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DOI: https://doi.org/10.1385/BTER:93:1-3:87