Abstract
By using an LKB2277 Bioactivity Monitor, stop-flow mode, the power-time curves of Escherichia coli at 37°C affected by Cu(II) were determined. Some parameters, such as growth rate constants k, inhibitory ratio I, the heat output Qlog in the log phase, and the generation times G were obtained. According to these parameters, we found that a low concentration of Cu(II) (0–20 µg/mL) had an promoting action on the growth of E. coli, but a high concentration of Cu(II) (40–100 µg/mL) had an inhibitory action. The toxicity of Cu(II) can also be expressed as the half-inhibitory concentration IC50; the value is 69.7 µg/mL. The assay is quantitative, inexpensive, and versatile.
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Yao, J., Liu, Y., Liu, P. et al. Study of the thermokinetic properties of copper(II) on Escherichia coli growth. Biol Trace Elem Res 92, 61–69 (2003). https://doi.org/10.1385/BTER:92:1:61
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DOI: https://doi.org/10.1385/BTER:92:1:61