Skip to main content
SpringerLink
Log in

Inhibition of glutathione reductase by cadmium ion in some rabbit tissues and the protective role of dietary selenium

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

This study is aimed at investigating the inhibitory effect of cadmium ion on glutathione reductase activity of rabbit brain and liver and the relationship of this effect with dietary selenium. For this purpose, one group of New Zealand rabbits were fed a selenium-deficient diet, another group was fed a selenium-rich diet, and the control group was fed a normal diet. The brain and liver tissues of these groups were investigated for the in vitro inhibitory effects of Cd2+ on glutathione reductase activity. For liver, the percentage inhibition of glutathione reductase by 40 nmol/mg protein of Cd2+ was similar for selenium-deficient and control groups, but significantly lower in the selenium-rich group. For brain tissues, there was no difference with respect to cadmium inhibition of glutathione reductase in all three groups.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. S. Shukla and R. L. Singhal, The present status of biological effects of toxic metals in the environment: lead, cadmium and manganese, Can. J. Physiol. Pharmacol. 62, 1015–1031 (1984).

    PubMed  CAS  Google Scholar 

  2. D. Manca, A. C. Ricard, B. Trottier, and G. Chevalier, Studies on lipid peroxidation in rat tissues following administration of low and moderate doses of cadmium chloride, Toxicology 67, 303–323 (1991).

    Article  PubMed  CAS  Google Scholar 

  3. E. Szymanska and T. Laskowska-Klita, Effect of cadmium (CdCl2) on lipid peroxidation and activities of antioxidant enzymes, Acta Biochim. Pol. 40, 144–146 (1993).

    PubMed  CAS  Google Scholar 

  4. M. T. Serafini, A. Romeu, and L. I. Arola, Zn(II), Cd(II) and Cu(II) interactions on glutathione reductase and glucose-6-phosphate dehydrogenase, Biochem. Int. 18, 793–802 (1989).

    PubMed  CAS  Google Scholar 

  5. N. L. Acan and E. F. Tezcan, Inhibition kinetics of sheep brain glutathione reductase by cadmium ion, Biochem. Mol. Med. 54, 33–37 (1995).

    Article  PubMed  CAS  Google Scholar 

  6. J. Neve, Physiological and nutritional importance of selenium, Experienta 47, 187–193 (1991).

    Article  CAS  Google Scholar 

  7. N. N. Ulusu, N. L. Acan, B. Turan, and E. F. Tezcan, The effect of selenium on glutathione redox cycle enzymes of some rabbit tissues, Trace Elements Electrolytes 17, 25–29 (2000).

    CAS  Google Scholar 

  8. B. Turan, N. L. Acan, N. N. Ulusu, and E. F. Tezcan, A comparative study on effect of dietary selenium and vitamin E on some antioxidant enzyme activities of some rabbit tissues, Biol. Trace Element Res. 81, 141–152 (2001).

    Article  CAS  Google Scholar 

  9. M. J. Christensen, A. L. Hancock, and A. H. Ford, Modifying effects of supplemental selenium and sulphur on cadmium tixicity in rats, Arch. Environ. Contam. Toxicol. 16, 717–722 (1987).

    Article  PubMed  CAS  Google Scholar 

  10. S. Sarkar, Y. Poonam, and D. Bhatnagar, Cadmium-induced lipid peroxidation and the antioxidant enzymes in rat tissues: role of vitamin E and selenium, Trace Elements Electrolytes 14, 41–45 (1997).

    CAS  Google Scholar 

  11. S. J. Yiin, C. L. Chern, J. Y. Sheu, and T. H. Lin, Cadmium-induced liver, heart and spleen lipid peroxidation in rats and protection by selenium, Biol. Trace Element Res. 78, 219–230 (2000).

    Article  CAS  Google Scholar 

  12. N. L. Acan and E. F. Tezcan, Sheep brain glutathione reductase: purification and general properties, FEBS Lett. 250, 72–74 (1989).

    Article  PubMed  CAS  Google Scholar 

  13. M. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding, Anal. Biochem. 72, 248–254 (1976).

    Article  PubMed  CAS  Google Scholar 

  14. K. Sumbuloglu, Saglik Bilimlerinde Arastirma Teknikleri ve Istatistik, Matis, Ankara, pp. 146–150 (1978).

    Google Scholar 

  15. N. N. Ulusu, N. L. Acan, B. Turan, and E. F. Tezcan, Effect of cadmium ion on glutathione reductase and glutathione peroxidase activity of rat liver and the relationship with dietary selenium, Biokim. Derg. 27, 43–46 (2002).

    CAS  Google Scholar 

  16. M. Bjornstedt, S. Kumar, L. Bjorkhem, G. Spyrou, and A. Holmgren, Selenium and thioredoxin and glutaredoxin systems, Biomed. Environ. Sci. 10, 271–279 (1997).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Hacettepe University, Faculty of Medicine, Ankara, Turkey

    N. Nuray Ulusu, N. Leyla Acan & E. Ferhan Tezcan

  2. Department of Biophysics, Ankara University, Faculty of Medicine, Ankara, Turkey

    Belma Turan

Authors
  1. N. Nuray Ulusu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Leyla Acan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Belma Turan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Ferhan Tezcan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ulusu, N.N., Acan, N.L., Turan, B. et al. Inhibition of glutathione reductase by cadmium ion in some rabbit tissues and the protective role of dietary selenium. Biol Trace Elem Res 91, 151–156 (2003). https://doi.org/10.1385/BTER:91:2:151

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/BTER:91:2:151

Index Entries

Search

Navigation