Skip to main content
SpringerLink
Log in

Antimutagenic activity of selenium-enriched green tea toward the heterocyclic amine 2-amino-3-methylimidazo[4,5-f]quinoline

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Both selenium and green tea have been reported to exhibit antigenotoxic and cancer chemopreventive properties. We compared the antimutagenic activities of regular green tea and selenium-enriched green tea obtained from Hubei Province, China, toward the heterocyclic amine, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in the Salmonella assay. Selenium-enriched green tea obtained by foliar application of selenite exhibited concentration-dependent inhibition of IQ-induced mutagenesis in the presence of rat liver S9 and was significantly more effective than regular green tea tested under the same conditions. Analytical studies revealed no major differences in the polyphenol or caffeine content between regular green tea and selenium-enriched green tea, but the latter tea contained approximately 60-fold higher concentrations of selenium compared with regular green tea. The only soluble form of selenium was identified as selenite. The antimutagenic effects of certain individual tea constituents, such as epicatechin gallate and catechin, were enhanced by the addition of selenite to the Salmonella assay. Sodium selenite, sodium selenate, seleno-dl-cysteine, seleno-l-methionine, and l-Se-methylselenocysteine were not antimutagenic toward IQ when tested alone, but augmented significantly the inhibitory potency of green tea. The results suggested an enhancing (“coantimutagenic”) effect of selenium in combination with green tea in vitro, but in vivo studies are needed to assess whether there is a synergistic effect of tea and selenium to protect against heterocyclic amine-induced mutagenesis and carcinogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L. B. M. Tijburg, T. Mattern, J. D. Folts, U. M. Wiesgerber, and M. B. Katan, Teaflavonoids and cardiovascular diseases: a review, Crit. Rev. Food Sci. Nutr. 37, 761–770 (1997).

    Article  Google Scholar 

  2. H. Mukhtar and N. Ahmad, Green tea in chemoprevention of cancer, Toxicol. Sci. 52, 111–117 (1999).

    PubMed  CAS  Google Scholar 

  3. Z. Y. Wang, R. Agarwal, W. A. Khan, and H. Mukhtar, Protection against benzo[a]pyrene and N-nitrosodiethylamine-induced lung and forestomach tumorigenesis in A/J mice by water extracts of green tea and licorice, Carcinogenesis 8, 1491–1494 (1992).

    Article  Google Scholar 

  4. M. Xu, A. C. Bailey, J. F. Hernaez, C. R. Taoka, H. A. J. Schut, and R. H. Dashwood, Protection by green tea, black tea, and indole-3-carbinol against 2-amino-3-methylimidazo[4,5-f]quinoline-induced DNA adducts and colonic aberrant crypts in the F344 rat, Carcinogenesis 17, 1429–1434 (1996).

    Article  PubMed  CAS  Google Scholar 

  5. M. Hirose, S. Takahashi, K. Ogawa, M. Futakuchi, and T. Shirai, Phenolics: blocking agents for heterocyclic amine-induced carcinogenesis, Food Chem. Toxicol. 37, 985–992 (1990).

    Article  Google Scholar 

  6. H. A. J. Schut and R. Yao, Tea as a potential chemopreventive agent in PhIP carcinogenesis: effects of green tea and black tea on PhIP-DNA adduct formation in female F-344 rats, Nutr. Cancer 36, 52–58 (2000).

    Article  PubMed  CAS  Google Scholar 

  7. G. Santana-Rios, G. A. Orner, A. Amantana, C. Provost, S-Y. Wu, and R. H. Dashwood, Potent antimutagenic activity of white tea in comparison with green tea in the Salmonella assay, Mutat. Res. 495, 61–74 (2001).

    PubMed  CAS  Google Scholar 

  8. T. Sugimura, Overview of carcinogenic heterocyclic amines, Mutat. Res. 376, 211–219 (1997).

    PubMed  CAS  Google Scholar 

  9. G. Santana-Rios, G. A. Orner, M. Xu, M. Izquerdo-Pulido, and R. H. Dashwood, Inhibition by white tea of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-induced colonic aberrant crypts in the F344 rat, Nutr. Cancer, in press (2002).

  10. E. G. Snyderwine, P. J. Wirth, P. P. Roller, R. H. Adamson, S. Sato, and S. S. Thorgeirsson, Mutagenicity and in vitro covalent DNAbinding of 2-hydroxyamino-3-methylimidazo[4,5-f]quinoline, Carcinogenesis 9, 411–418 (1998).

    Article  Google Scholar 

  11. W. W. Huber, L. P. McDaniel, K. R. Kaderlik, C. H. Teitel, N. P. Lang, and F. F. Kadlubar, Chemoprotection against the formation of colon DNA adducts from the food-borne carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in the rat, Mutat. Res. 376, 115–122 (1997).

    PubMed  CAS  Google Scholar 

  12. C. P. Nelson, R. K. La Creis, J. Sauvageot, W. B. Isaacs, A. M. De Marzo, J. D. Groopman, et al., Protection against 2-hydroxyamino-1-methyl-6-phenylimi-dazo[4,5-b]pyridine cytotoxicity and DNA adduct formation in human prostate by glutathione S-transferase, Cancer Res. 61, 103–109 (2001).

    PubMed  CAS  Google Scholar 

  13. H. Hayatsu, N. Inada, T. Kakutani, S. Arimoto, T. Negishi, K. Mori, et al. Suppression of genotoxicity of carcinogens by (-)-epigallocatechin gallate, Prev. Med. 21, 370–376 (1992).

    Article  PubMed  CAS  Google Scholar 

  14. J. Hernaez, M. Xu, and R. H. Dashwood, Effects of tea and chlorophyllin on the mutagenicity of N-hydroxy-IQ: studies of enzyme inhibition, molecular complex formation, and degradation/scavenging of the active metabolites, Environ. Mol. Mutagen. 30, 468–474 (1997).

    Article  PubMed  CAS  Google Scholar 

  15. J. F. Hernaez, M. Xu, and R. H. Dashwood, Antimutagenic activity of tea towards 2-hydroxyamino-3-methylimidazo[4,5-f]quinoline: effect of tea concentration and brew time on electrophile scavenging, Mutat. Res. 402, 299–306 (1998).

    PubMed  CAS  Google Scholar 

  16. R. J. Shamberger and C. E. Willis, Selenium distribution of human cancer mortality, CRC Crit. Rev. Clin. Lab. Sci. 2, 211–219 (1971).

    Article  PubMed  CAS  Google Scholar 

  17. L. Clark, L. Hixon, J. F. Combs, M. Reid, B. W. Turnbull, and R. Sampliner, Plasma selenium concentration predicts the prevalence of colorectal adenomatous polyps. Cancer Epidemiol. Biomarkers Prev. 2, 41–46 (1993).

    PubMed  CAS  Google Scholar 

  18. L. C. Clark, J. F. Combs, B. W. Turnbull, E. H. Slate, D. K. Chalker, J. Chow, et al., Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin, J. Am. Med. Assoc. 276, 1957–1985 (1966).

    Article  Google Scholar 

  19. C. Ip, Lessons from basic research in selenium and cancer prevention, J. Nutr. 128, 1845–1854 (1998).

    PubMed  CAS  Google Scholar 

  20. Y. Feng, J. W. Finley, C. D. Davis, W. K. Becker, A. J. Fretland, and D. W. Hein, Dietary selenium reduces the formation of aberrant crypts in rats administered 3,2′-dimethyl-4-aminobiphenyl, Toxicol. Appl. Pharmacol. 157, 36–42 (1999).

    Article  PubMed  CAS  Google Scholar 

  21. A. T. Baines, H. Holubec, J. L. Basye, P. Thorne, A. K. Bhattacharyya, J. Spallholz, et al. The effects of dietary selenomethionine on polyamines and azoxymethane-induced aberrant crypts, Cancer Lett. 160, 193–198 (2000).

    Article  PubMed  CAS  Google Scholar 

  22. B. S. Reddy, Y. Hirose, R. A. Lubet, V. E. Steele, G. J. Kelloff, and C. V. Rao, Lack of chemopreventive efficacy of DL- selenomethionine in colon carcinogenesis. Int. J. Mol. Med. 5, 327–330 (2000).

    PubMed  CAS  Google Scholar 

  23. C. V. Rao, I. Coomal, J. G. Rodriguez, B. Simi, K. El-Bayoumy, and B. S. Reddy, Chemoprevention of familial adenomatous polyposis development in the APC(min) mouse model by 1,4-phenylene bis(methylene)selenocyanate, Carcinogenesis 21, 617–621 (2000).

    Article  PubMed  CAS  Google Scholar 

  24. M. Rizki, S. Amrani, A. Creus, N. Xamena, and R. Marcos, Antigenotoxic properties of selenium: studies in the Wing Spot Test of Drosophila, Environ. Mol. Mutagen. 37, 70–75 (2001).

    Article  PubMed  CAS  Google Scholar 

  25. A. Amantana, G. Santana-Rios, J. A. Butler, P. D. Whanger, and R. H. Dashwood, Antimutagenic activity of selenium-enriched green tea towards a cooked meat mutagen, FASEB J. 14, A537 (2000).

    Google Scholar 

  26. M. A. Beilstein and P. D. Whanger, Deposition of dietary organic and inorganic selenium in rat erythrocyte proteins, J. Nutr. 116, 1701–1710 (1986).

    PubMed  CAS  Google Scholar 

  27. Kotrebai, M. Birringer, J. F. Tyson, E. Block, and P. C. Uden, Identification of the principal selenium compounds in selenium-enriched natural sample extracts by ion-pair liquid chromatography with inductively coupled plasma- and electrospray ionization-mass spectrometric detection, Anal. Commun. 36, 249–252 (1999).

    Article  CAS  Google Scholar 

  28. G. F. Combs and S. B. Combs, The role of selenium in nutrition, in Chemical Aspects of Selenium, Academic, Orlando, FL, 1st edition, Chap. 1 (1986).

    Google Scholar 

  29. X. J. Cai, E. Block, P. C. Uden, X. Zhang, B. D. Quimby, and J. J. Sullivan, Allium chemistry: identification of selenoamino acids in ordinary and selenium-enriched garlic, onions, and broccoli using gas chromatography with atomic emission detection, J. Agric. Food Chem. 43, 1754–1757 (1995).

    Article  CAS  Google Scholar 

  30. P. D. Whanger, C. Ip, C. E. Polan, P. C. Uden, and G. Welbaum, Tumorigenesis, metabolism, speciation, bioavailability and tissue deposition of selenium in seleniumenriched ramps (Allium tricoccum), J. Agric. Food Chem. 48, 5723–5730 (2000).

    Article  PubMed  CAS  Google Scholar 

  31. J. W. Finley, C. D. Davis, and Y. Feng, Selenium from high-selenium broccoli protects rats from colon cancer, J. Nutr. 130, 2384–2389 (2000).

    PubMed  CAS  Google Scholar 

  32. J. W. Finley, C. Ip, D. J. Lish, C. D. Davis, K. J. Hintze, and P. D. Whanger, Cancerprotective properties of high selenium broccoli, J. Agric. Food Chem. 49, 2679–2683 (2001).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Environmental and Molecular Toxicology, Oregon State University, 97331, Corvallis, OR

    Adams Amantana, Judith A. Butler, Phil D. Whanger & Roderick H. Dashwood

  2. The Linus Pauling Institute, Oregon State University, 97331, Corvallis, OR

    Gilberto Santana-Rios, Meirong Xu & Roderick H. Dashwood

Authors
  1. Adams Amantana
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gilberto Santana-Rios
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Judith A. Butler
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Meirong Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Phil D. Whanger
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Roderick H. Dashwood
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Amantana, A., Santana-Rios, G., Butler, J.A. et al. Antimutagenic activity of selenium-enriched green tea toward the heterocyclic amine 2-amino-3-methylimidazo[4,5-f]quinoline. Biol Trace Elem Res 86, 177–191 (2002). https://doi.org/10.1385/BTER:86:2:177

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/BTER:86:2:177

Index Entries

Search

Navigation