Abstract
The effects of Al(III) on surface properties and lactate accumulation by Bifidobacterium thermophilum were investigated. Bacteria were treated with Al(III) at 37°C and 4°C, then exposed to free radicals or nisin. When exposed to Al(III) at 37°C, the organism exhibited spreading on hydrophobic surfaces and showed high susceptibility to free-radical alteration as indicated by Fe(III) binding, but showed little effect on lactate production in the presence or absence of nisin, even after washing with 2 mM EDTA. At 4°C, there was no increased surface spreading or binding of Fe(III), but protection against nisin action was present. This, however, was abolished after washing with EDTA. It was concluded that membrane fluidity is required to affect membrane lipid rearrangement, resulting in surface spreading and increased susceptibility to peroxidation, whereas only loose binding of Al(III) to membrane surfaces is sufficient to prevent transmembrane channel formation by nisin.
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References
M. E. Sanders, Overview of functional foods. Emphasis on probiotic bacteria, Int. Dairy J. 8, 507–512 (1998).
R. Fuller, Probiotics for farm animalas, in Probiotics. A Critical Review, G. W. Tannock, ed., Horizon Scientific, Norfolk, VA, pp. 15–22 (1999).
A. Bezkorovainy, Probiotics: determinants of survival and growth in the gut, Am. J. Clin. Nutr. 73(Suppl.), 399S-405S (2001).
A. Bezkorovainy and L. Solberg, Ferrous iron uptake by Bifidobacterium breve, Biol. Trace Element Res. 20, 251–267 (1989).
E. Kot and A. Bezkorovainy, Binding of ferric iron to the cell walls and membranes of Bifidobacterium thermophilum: effect of free radicals, J. Agric. Food Chem. 47, 4606–4610 (1999).
E. Kot and A. Bezkorovainy, The binding of H2O2-generated Fe(III) to Bifidobacterium thermophilum depends on the method of its preparation, J. Food Sci. 65, 1028–1032 (2000).
P. Oteiza, A mechanism for the stimulation effect of aluminum on iron-induced lipid peroxidation, Arch. Biochem. Biophys. 308, 374–379 (1994).
E. Kot and A. Bezkorovainy, The effect of nisin on the physiology of Bifidobacterium thermophilum, J. Food Protect. 68, 1206–1210 (2001).
T. J. Montville and Y. Chen, Mechanistic action of pediocin and nisin: recent progress and unresolved questions, Appl. Microbiol. Biotechnol. 50, 511–519 (1998).
V. Scardovi, Genus Bifidobacterium Orla Jensen 1924, 472, in Bergey’s Manual of Systematic Bacteriology Vol. 2, P. Sneath, ed., Williams & Wilkins, Baltimore, MD, pp. 1418–1434 (1986).
P. Brigidi, D. Mateuzzi, and F. Crociani, Protoplast formation and regeneration in Bifidobacterium, Microbiologica 9, 243–250 (1986).
E. Kot, R. Miller-Catchpole, and A. Bezkorovainy, Iron uptake by Bifidobacterium thermophilum protoplasts, Biol. Trace Element Res. 38, 1–12 (1993).
O. Lombard, Difference between means, in Biostatistics for the Health Professions, Appleton, Century, Krofts, New York, pp. 71–77 (1975).
T. M. Devlin, Biological membranes: structure and membrane transport, in Textbook of Biochemistry, T. M. Devlin, ed., Wiley-Liss, New York, p. 194 (1997).
F. Katz, Active cultures add function to yogurt and other foods, Food Technol. 55, 46–49 (2001).
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Kot, E., Bezkorovainy, A. Effect of Al(III) on surface properties of Bifidobacterium thermophilum as a function of temperature. Biol Trace Elem Res 86, 159–165 (2002). https://doi.org/10.1385/BTER:86:2:159
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DOI: https://doi.org/10.1385/BTER:86:2:159