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Binding of mineral elements to locust bean gum influences availability in vitro

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Abstract

Experiments were conducted to evaluate the extent to which element binding of locust bean gum (LBG) affects the availability of calcium, iron, and zinc in the gut. Infant formula was supplemented with increasing amounts of LBG and subjected to an intraluminal digestion procedure. Element binding was measured by eliminating the complexes by twofold centrifugation. Availability of the elements was determined using a validated continuous-flow dialysis technique. Elemental content of the samples, supernatants, and dialysates was analyzed with validated atomic absorption spectrometry. LBG provided small amounts of intrinsic calcium (1.13 ± 0.02 mg/g) and trace amounts of iron (0.02 ± 0.00 mg/g) and zinc (0.01 ± 0.00 mg/g), which were strongly bound to the LBG molecule (respectively: 76.6 ± 3.3%, 83.4 ± 1.2%, 96.7 ± 6.6%). Correlation analysis, between percent element bound by LBG after centrifugation and percent trapped after dialysis, yielded significant correlation only for the data of zinc (r=0.93). For calcium and iron, no correlation could be demonstrated; however, for iron a similar trend was observed. These findings suggest that element binding of LBG has a major influence on the availability of zinc and maybe of iron. For calcium, other factors might also be involved, affecting availability.

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References

  1. A. E. Bender, Nutritional significance of bioavailability, in Nutrient Bioavailability: Chemical and Biological Aspects, D. Southgate, I. Johnson, and G. R. Fenwick, eds., Royal Society of Chemistry, Cambridge, pp. 3–9 (1989).

    Google Scholar 

  2. S. J. Fairweather-Tait, Trace element bioavailability, in Role of Trace Elements for Health Promotion and Disease Prevention, B. Sandström and P. Walter, eds., Karger, Basel, pp. 29–39 (1998).

    Chapter  Google Scholar 

  3. D. Bosscher, K. Van Dyck, H. Robberecht, M. Van Caillie-Bertrand, and H. Deelstra, Bioavailability of calcium and zinc from cow’s milk-based versus soya-based infant food, Int. J. Food Sci. Nutr. 49, 277–283 (1998).

    Article  CAS  Google Scholar 

  4. K. Van Dyck, S. Tas, H. Robberecht, and H. Deelstra, The influence of different food components on the in vitro availability of iron, zinc and calcium from a composed meal, Int. J. Food Sci. Nutr. 47, 499–506 (1996).

    PubMed  Google Scholar 

  5. R. M. Forbes and J. W. Erdman, Bioavailability of trace mineral elements, Annu. Rev. Nutr. 3, 213–231 (1983).

    Article  PubMed  CAS  Google Scholar 

  6. D. B. Clayton, Complex Carbohydrates in Foods, Chapman & Hall, London (1990).

    Google Scholar 

  7. D. Bosscher, M. Van Caillie-Bertrand, K. Van Dyck, H. Robberecht, R. Van Cauwenbergh, and H. Deelstra, Thickening infant formulas with digestible and indigestible carbohydrate: availability of calcium, iron, and zinc in vitro, J. Pediatr. Gastroenterol. Nutr. 30, 373–378 (2000).

    Article  PubMed  CAS  Google Scholar 

  8. L. A. Persson, M. Lundström, B. Lönnerdal, and O. Hernell, Are weaning foods causing impaired iron and zinc status in 1-year-old Swedish infants? A cohort study, Acta Pædiatr. 87, 618–622. (1998).

    Article  PubMed  CAS  Google Scholar 

  9. Nationale raad voor de voeding, Voedingsaanbevelingen voor België, G. De Backer, Sint-Martens-Latem (1996).

  10. P. Hendrix, R. Van Cauwenbergh, H. Robberecht, and H. Deelstra, Daily dietary zinc intake in Belgium measured using duplicate portion sampling, Z. Lebensm. Unters. Forsch. 206, 222–227 (1998).

    Article  CAS  Google Scholar 

  11. D. Bosscher, M. Van Caillie-Bertrand, H. Robberecht, K. Van Dyck, R. Van Cauwenbergh, and H. Deelstra, In vitro availability of calcium, iron and zinc from first-age infant formula and human milk, J. Pediatr. Gastroenterol. Nutr., 32, 54–58 (2001).

    Article  PubMed  CAS  Google Scholar 

  12. I. Furda, in Dietary fibres chemistry and nutrition, G. E. Inglett and S. I. Falkenberg, eds., Academic Press, New York, pp. 31–48 (1979).

    Google Scholar 

  13. B. M. Nair, N. Asp, M. Nyman, and H. Persson, Binding of mineral elements by some dietary fibre components—in vitro, Food Chem. 23, 295–303 (1987).

    Article  CAS  Google Scholar 

  14. L. A. Berner and F. H. Lamartine, Iron binding by sodium alginate, J. Food Sci. 48, 755–758 (1983).

    Article  CAS  Google Scholar 

  15. A. Harmuth-Hoene, A. Meier-Ploeger, and C. Leitzmann, Der einfluss von Johannisbrotkernmehl auf die resorption von Mineralstoffen und spurenelementen beim Menschen, Z. Ernahrungswiss. 21, 202–213 (1982).

    Article  PubMed  CAS  Google Scholar 

  16. J. A. Rendleman, Metal-polysaccharide complexes—Part II, Fundam. Chem. 3, 127–162 (1978).

    CAS  Google Scholar 

  17. S. R. Platt and F. M. Clydesdale, Mineral binding characteristics of lignin, guar gum, cellulose, pectin and neutral detergent fiber under simulated duodenal pH conditions, J. Food Sci. 52, 1414–1419 (1987).

    Article  CAS  Google Scholar 

  18. R. Fernandez and S. F. Phillips, Components of fibre bind iron in vitro, Am. J. Clin. Nutr. 35, 100–106 (1982).

    PubMed  CAS  Google Scholar 

  19. A. Harmuth-Hoene and R. Schelenz, Effect of dietary fiber on mineral absorption in growing rats, J. Nutr. 110, 1774–1784 (1980).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Laboratory of Food Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp (Wilrijk), Belgium

    Douwina Bosscher, Harry Robberecht, Rudy Van Cauwenbergh & Hendrik Deelstra

  2. Department of Pediatric Gastroenterology and Nutrition, Koningin Paola Kinderziekenhuis, Algemeen Ziekenhuis Middelheim, Lindendreef 1, B-2020, Antwerp, Belgium

    Micheline Van Caillie-Bertrand

Authors
  1. Douwina Bosscher
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  2. Harry Robberecht
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  3. Rudy Van Cauwenbergh
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  4. Micheline Van Caillie-Bertrand
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  5. Hendrik Deelstra
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Bosscher, D., Robberecht, H., Van Cauwenbergh, R. et al. Binding of mineral elements to locust bean gum influences availability in vitro. Biol Trace Elem Res 81, 79–92 (2001). https://doi.org/10.1385/BTER:81:1:79

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  • DOI: https://doi.org/10.1385/BTER:81:1:79

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