Abstract
Hypokinesia (diminished movement) induces significant calcium (Ca) changes, but little is known about the effect of hypokinesia (HK) on Ca deficiency. Measuring Ca changes during and after HK the aim of this study was to determine Ca deficiency during prolonged HK.
Studies were done on 12 male Macaca mulatta (rhesus monkeys) aged 3–5 yr (5.58–6.42 kg) during a 90-d pre-HK period, a 90-d HK period, and a 15-d post-HK period. Monkeys were equally divided into two groups: vivarium control monkeys (VCM) and hypokinetic monkeys (HKM). Hypokinetic monkeys were kept in small individual cages that restricted their movements in all directions without hindering food and water intakes.
Urinary, fecal, and serum Ca, urinary and serum magnesium (Mg) and phosphate (P), serum intact parathyroid hormone (iPTH), and calcitonin (CT) concentration, body weight, food intake, fluid consumed and eliminated in urine were measured. During the HK period, fecal Ca loss, urinary Ca, P, and Mg excretion, fluid elimination, and serum P, Ca, and Mg concentration increased significantly (p≤0.01), whereas serum iPTH and CT concentration, food and fluid intakes, and body weight decreased significantly (p≤0.01) in the HKM group when compared with the VCM group. During the initial days of the post-HK period, serum Ca, Mg, and P concentration, fecal Ca loss, urinary Ca, Mg, and P excretion, and fluid elimination decreased significantly (p≤0.01), whereas fluid intake increased significantly (p≤0.01) in the HKM group when compared with the VCM group. Food intake, body weight, and serum iPTH and CT concentrations remained significantly (p≤0.01) depressed in the HKP group when compared with the VCM; however, they increased as the duration of the post-HK period increased. By contrast, the corresponding parameters remained stable in the VCM group when compared with the baseline control values.
It was shown that fecal and urinary Ca loss and serum Ca concentration increases significantly during HK, whereas during post-HK fecal, urinary, and serum Ca decreases significantly. It was concluded that significant decrease of serum, urinary, and fecal Ca during post-HK may suggest the presence of Ca deficiency during prolonged HK.
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References
A. V. Volozhin, Pathogenesis of disturbances of calcium metabolism in mineralized tissues during prolonged hypokinesia, Ph.D. thesis, Academy of Sciences USSR and Directorate of Kosmic Biology and Medicine, Ministry of Health USSR, Moscow (1978).
V. P. Krotov, Kinetics and regulation of fluid and electrolyte metabolism in animals and human beings during prolonged hypokinesia, Ph.D. thesis, Academy of Sciences USSR, Ministry of Health USSR, Moscow (1978).
A. I. Grigor’yev, B. R. Dorokhova, V. Yu. Semenov, B. V. Morukov, E. O. Baychorov, I. S. Skukina, and B. V. Afonin, Fluid-electrolyte metabolism and renal function in kosmonauts following 185-day Kosmic flight, Kosmicheskaya Biol. 19, 21–27 (1985).
A. I. Grigor’yev, B. R. Dorokhova, G. S. Arzamazov, and B. V. Morukov, Electrolyte regulating function of human kidneys during long-term Kosmic flights and simulating weightlessness conditions, Kosmicheskaya Biol. 16, 29–33 (1982).
G. Zorbas, Y. F. Federenko, and K. A. Naexu, Calcium loading and renal function in trained subjects during restriction of muscular activity and chronic hyperhydration, Biol. Trace Element Res. 41, 137–156 (1994).
Y. G. Zorbas, Y. F. Federenko, and K. A. Naexu, Bone mineralization and plasma concentration of electrolytes in healthy subjects after exposure to hypokinesia and hyperhydration, Wiener Klin. Wochenschr. 105, 167–171 (1993).
N. Krupina, G. P. Mikhaylovskiy, and A. Ya. Tizul, Adaptation of Muscular Activity and Hypokinesia, Meditsina, Novosibirsk (1970).
Ye. A. Kovalenko, E. S. Mailyan, and V. L. Popkov, Advances in Physiological Sciences, Meditsina, Moscow (1975).
Y. G. Zorbas and V. R. Bobylev, General resistance of organisms of rats under hypokinesia, Proceedings 2nd European Symposium on Life Sciences Research in Space, Poz-Wahn, Germany, pp. 203–206 (1984).
I. V. Fedorov, V. N. Vinogradov, Yu. I. Milov, and L. A. Grishanina, Synthesis of tissue proteins in animals during hypodynamia, Kosmicheskaya Biol. 1, 53–57 (1967).
I. V. Fedorov, Intensity of tissue autolysis in animal during hypodynamia, Kosmicheskaya Biol. 5, 82–84 (1971).
I. V. Fedorov, A. V. Chernyy, and A. I. Fedorov, Synthesis and catabolism of tissue proteins during hypodynamia and resumption of muscular activity, Fiziol. Zh. SSR 63, 1128–1133 (1977).
Y. G. Zorbas, G. E. Verentsov, and Y. F. Federenko, Renal excretion of end products of protein metabolism in urine of endurance trained subjects during prolonged restriction of muscular activity, Panminerva Med. 37, 109–119 (1995).
Y. G. N. B. Alexeyev and Z. A. Nikolescu, Water mineral metabolism in muscles and tissues of rabbits under hypokinesia, Mater. Med. Polona 22, 267–273 (1990).
Y. G. Zorbas, A. L. Ivanov, and Y. F. Fujiyama, Electrolyte content in organs and tissues of rats during and after hypokinesia, Mater. Med. Polona 22, 263–266 (1990).
Y. G. Zorbas, A. L. Ivanov, and Y. N. Fujiyama, Electrolyte composition of skeletal muscles after hypokinesia, Mater. Med. Polona 22, 286–288 (1990).
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Zorbas, Y.G., Kakurin, V.J., Afonin, V.B. et al. Calcium measurements in primates during and after hypokinesia in establishing calcium deficiency during prolonged hypokinesia. Biol Trace Elem Res 76, 113–131 (2000). https://doi.org/10.1385/BTER:76:2:113
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DOI: https://doi.org/10.1385/BTER:76:2:113