Abstract
Many factors are known to influence trace element metabolism and one of them is dietary protein. The present study examines the effects of casein, soybean protein, and peanut protein on the metabolism of the Zn, Cu, Fe, and Mn in growing rats. The results showed that Zn, Fe, and Mn excretions in the feces of peanut protein-fed rats (PPERs) were similar to that of casein-fed rats (CPFRs) (p>0.05), whereas all of the Zn, Cu, Fe, and Mn excretions in the urine of PPFRs were significantly higher than that of CPFRs (p<0.05), but its apparent absorption rate (AAR) of Cu, Fe and its apparent retention rate (ARR) of Cu were all higher than that of CPFRs (p<0.05). Hepatic Zn content of soybean protein-fed rats (SPFRs) was higher than that of CPFRs and PPFRs (p<0.05 respectively) and serum, renal, and femoral Cu contents of SPFRs were significantly lower; however, hepatic Cu, and renal Mn contents were significantly higher than that of CPFRs (p<0.05, respectively); The hepatic Fe content of SPFRs was significantly higher than that of CPFRs and PPFRs (p<0.01, respectively). To sum up, compared to casein, soybean protein might be a good dietary source to make up for Zn and Fe deficiency, and also peanut protein to make up for Cu and Fe deficiency.
Similar content being viewed by others
References
J. L. Greger and S. M. Snedeker, Effect of dietary protein and phosphorus levels on the utilization of zinc, copper and manganese by adult males, J. Nutr. 110, 2243–2253 (1980).
S. B. Zhou, G. J. Wang, S. Y. Yu, and C. Y. Bao, The effect of different protein intake levels on the utilization of protein, zinc, iron and copper in rats, Acta Nutr. Sin. 17, 135–140 (1995).
H. Y. Neggers and S. Jarnail, Effect of dietary protein, zinc, concentration in mice, Biol. Trace Element Res., 98, 171–179 (2004).
C. Wang, Y. Q. Guan, X. M. Hu, and X. Z. Wen, Study on relation of different proteins and zinc metabolism in rats, Chin. J. Food Hyg. 15, 305–307 (2003).
G. R. Philip, H. N. Forrest, and C. F. George, AIN-93G purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet, J. Nutr. 123, 1939–1951 (1993).
B. Sandstrom, A. Almgren, and B. Kivisto, Effect of protein level and protein source on zinc absorption in human, Nutrition 119, 48 (1989).
R. A. Wapnir, Copper absorption and bioavailability, Am. J. Clin. Nutr. 67(Suppl.), 1054–1060 (1998).
W. Ingeborg, F. B. Xandra, and L. Egidius, Liver copper content of rats hypo- or hyper-responsive to dietary cholesterol J. Trace Elements Med. Biol. 17, 177–182 (2002).
M. C. Linder and M. Roboz, Turnover and excretion of copper in rats as measured with 67Cu, Am. J. Physiol. 251, 551–555 (1986).
L. G. Strause, J. Hegenauer, and P. Saltman, Effects of long-term dietary manganese and copper deficiency on rat skeleton, J. Nutr. 116, 135–141 (1986).
R. Uauy, M. Olivares, and M. Conzalez, Essentiality of copper in humans, Am. J. Clin. Nutr. 67(Suppl.), 952–959 (1998).
C. D. Yee, S. K. Karen, and M. Walker, The relationship of nutritional copper to the development of postmenopausal osteoporosis in rats, Biol. Trace Element Res. 48, 1–11 (1995).
F. E. Viteri, The consequence of iron deficiency and anemia in pregnancy on maternal health the foetus and the infant, Sci. News 11, 14–17 (1994).
FAO, Assessment of the prevalence and causes of anemia, in Agriculture, Food and Nutrition for Africa, FAO, Rome, pp. 296–304 (1997).
F. Wedler, Biochemical and nutritional role of manganese: an overview, in Manganese in Health and Disease, D. Klimis-Tavantzis, ed., CRC, Boca Raton, FL, pp. 1–38 (1994).
J. W. Finley, P. E. Johnson, and L. K. Johnson, Sex affects manganese absorption and retention by humans from a diet adequate in manganese, Am. J. Clin. Nutr. 60, 949–955 (1994).
C. Davis, T. Wolf, and J. Greger, Varying levels of manganese and iron affect absorption and gut endogenous losses of manganese by rats, J. Nutr. 122, 1300–1308 (1992).
D. Y. Lee and P. E. Johnson, Factors affecting absorption and excretion of 54Mn in rats, J. Nutr. 118, 1509–1516 (1988).
L. Davidsson, A. Almgren, M. A. Juillerat, and R. F. Hurrell, Manganese absorption in humans: the effect of phytic acid and ascorbic acid in soybean formula, Am. J. Clin. Nutr. 62, 984–987 (1995).
R. N. Wapnir, Protein Nutrition and Mineral Absorption, CRC, Boca Raton, FL (1990).
K. J. Wedekind, E. C. Titgemeyer, A. R. Twardock, and D. H. Baker, Phosphorus, but not calcium, affects manganese absorption and turnover in chicks, J. Nutr. 121, 1776–1786 (1991).
L. Davidsson, A. Cederblad, B. Lönnerdal, and B. Sandstrom, The effect of individual dietary components on manganese absorption in humans, Am. J. Clin. Nutr. 54, 1065–1070 (1991).
F. R. Hurrell, Influence of vegetable protein sources on trace element and mineral bioavailability, J. Nutr. 133, 2973–2977 (2003).
J. R. Turnlund, J. C. King, B. Gong, and M. C. Michel, A stable isotope study of copper absorption in young man: effects of phytate and alpha-cellulose, Am. J. Clin. Nutr. 42, 18–23 (1985).
W. F. John, Manganese absorption and retention by young women is associated with serum ferritin concentration, Am. J. Clin. Nutr. 70, 37–43 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, G., Zhao, L., Bao, S. et al. Effects of different proteins on the metabolism of Zn, Cu, Fe, and Mn in rats. Biol Trace Elem Res 113, 165–175 (2006). https://doi.org/10.1385/BTER:113:2:165
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/BTER:113:2:165