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Induction of metallothionein-like proteins by mercury and distribution of mercury and selenium in the cells of hepatopancreas and gill tissues in mussel Mytilus galloprovincialis

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Abstract

The binding of mercury (Hg) to metallothioneins (MTs) and the relation between Hg and selenium in supernatants of hepatopancreas and gill tissues of the common mussel Mytilus galloprovincialis (Lamarck, 1819) was investigated. The mussels were exposed to different Hg concentrations in laboratory conditions: 2.5 μgHg/L, 4 d exposure (short term) and 60 μgHg/L, 33 d exposure (long term). In addition, the results were compared to those found for mussels from nature (polluted and unpolluted region). In control and short-term-exposed mussles, the level of Hg extraction (cytosol) from hepatopancreas and gill cells was very low with respect to the total Hg concentrations in the corresponding tissues, around 10% in control and around 20% after exposure. As expected, Hg exposure was followed by Se increase. For Se, the levels of extraction were higher, around 20% in control and up to 50% (heaptopacrease) of 70% (gills) after exposure. In order to study the distribution of Hg and Se in the cells of these organs, the total Hg and Secconcentrations were analyzed in the subcellular fractions obtained after differential centrifugation. Although after exposure the concentrations of both element increased in all subcellular fractions, their percentages in particular fractions were lower or higher. In this study, the convincing binding of Hg to metallothionein-like proteins was perceived after long-term laboratory exposure (gills, heapatopancreas) and in wild mussels collected near industrial port (hepatopancreas). In latter case, we also detected the traces of Se bound to the MT fractions after size-exclusion chromatography.

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Authors and Affiliations

  1. Jozef Stefan Institute, Jamuva 39, 1111, Ljubljana, Slovenia

    Magda Tusek Znidaric, Ingrid Falnoga & Mirjana Skreblin

  2. National Institute of Biology, Vecna pot 111, 1000, Ljubljana, Slovenia

    Valentina Turk

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  1. Magda Tusek Znidaric
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  2. Ingrid Falnoga
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  3. Mirjana Skreblin
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  4. Valentina Turk
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Znidaric, M.T., Falnoga, I., Skreblin, M. et al. Induction of metallothionein-like proteins by mercury and distribution of mercury and selenium in the cells of hepatopancreas and gill tissues in mussel Mytilus galloprovincialis . Biol Trace Elem Res 111, 121–135 (2006). https://doi.org/10.1385/BTER:111:1:121

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  • DOI: https://doi.org/10.1385/BTER:111:1:121

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