Abstract
Selenium (Se), an essential trace element, is incorporated into seleno-proteins as selenocysteine using insertion machinery, including UGA codon and selenocysteine insertion sequence (SECIS) element in the 3 untranslated region (3′-UTR) of mRNA. To assess the biological effects of tumor cells exposed to the elevated, but nontoxic Se level on glutathione peroxidase (GP×1 [cellularar] and GP×3 [extracellular]) thioredoxin reductase (TrxR), and selenoprotein P (SeP) mRNA expression, we introduced a semiquantitative reverse transcription-polymerase chain reaction technique for each selenoprotein transcript using β-actin as a reference housekeeping gene in mouse fibroblasts (WEHI 164). Cell lines were cultured with 1.0, 2.5, and 5.0 ng of Se in 1 mL of medium for 3 and 7 d, apart from the control cell line with standard medium. It was found that Se exerts a statistically significant (p<0.05) effect only on GP×3 mRNA, referred to as the optical density (OD) ratio (GP×3/ β-actin). Moreover, the lowest Se level affected GP×3 mRNA expression more strongly than its highest concentrations. In an in vitro model applied in this study, GP×3 gene expression is most specific for Se supplementation.
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Reszka, E., Gromadzinska, J., Stanczyk, M. et al. Effect of selenium on expression of selenoproteins in mouse fibrosarcoma cells. Biol Trace Elem Res 104, 165–172 (2005). https://doi.org/10.1385/BTER:104:2:165
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DOI: https://doi.org/10.1385/BTER:104:2:165