Abstract
The kinetics (V max and K m ) of the erythrocyte Na+−H+ exchanger was studied in a group of 21 patients undergoing regular hemodialysis (HD) and in 21 control subjects. The activity of antioxidative enzymes—superoxide dismutase and glutathione peroxidase—as well as the concentrations of their cofactors—zinc, copper, and selenium—in plasma and in erythrocytes were determined. The thiobarbituric acid-reactive substances (TBARS) concentration served as an indicator of oxidative stress intensity in plasma and erythrocytes. It was found that in the control group the concentration of copper in erythrocytes was positively correlated with K m and V max. When the concentration of copper increased, the shape of the kinetic curve changed from sigmoidal to hyperbolic. In the control group, the concentration of zinc in erythrocytes also correlated with K m . However, the results obtained for the group of hemodialyzed patients were the opposite: when the erythrocyte concentration of copper increased, a K m decline was observed and the shape of the curve changed from hyperbolic to sigmoidal. In the group of hemodialyzed patients, we also found a positive correlation between K m and the concentration of selenium in erythrocytes, and a negative correlation between K m and erythrocyte TBARS.
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Bober, J., Kwiatkowska, E., Ciechanowski, K. et al. Do trace elements modify the activity of erythrocyte sodium-proton exchanger in hemodialyzed patients?. Biol Trace Elem Res 104, 107–120 (2005). https://doi.org/10.1385/BTER:104:2:107
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DOI: https://doi.org/10.1385/BTER:104:2:107