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Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture

Biological Trace Element Research volume 104, pages 57–69 (2005)Cite this article

Abstract

Temporal changes in mRNAs for metallothionein (MT) isoforms in subcultures of rat marrow mesenchymal stem cells (MSCs) after treatment with dexamethasone were investigated. Both MT-1 and MT-2 mRNA expression in the cultured MSCs with dexamethasone showed maximum levels at d 1, whereas ALP and osteocalcin mRNAs peaked at d 12. MT-3 mRNA was not detected in the cultured MSCs at any time. The expression level of MT-2 mRNA at d 1 was 9.4-fold higher than that of MT-1 mRNA. Finally, osteoblast differentiation and mineralization of MSCs at d 14 was inhibited by the addition of a common antisense oligonucleotide for both MT-1 and MT-2 in the culture medium during the first 4 d. The results suggest that the large amounts of MT-2 are produced in the early stage of subculture of MSCs, and this might regulate their differentiation.

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Author notes

  1. Takeshi Minami

    Present address: Department of Life Sciences, School of Science and Technology, Kinki University, 577-8502, Higashi-osaka, Japan

Affiliations

  1. Department of Public Health, Nara Medical University, 840 Shijo-cho, 634-8521, Kashihara City, Nara, Japan

    Yoshiko Dohi, Hideki Shimaoka & Kunio Yonemasu

  2. Department of Oral and Maxillofacial Surgery, Nara Medical University, 840 Shijo-cho, 634-8521, Kashihara City, Nara, Japan

    Masako Ikeuchi

  3. Department of Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, Amagasaki Site 3-11-46 Nakouji, 661-0974, Amagasaki City, Hyogo, Japan

    Hajime Ohgushi

Authors
  1. Yoshiko Dohi
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  2. Hideki Shimaoka
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  3. Masako Ikeuchi
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  4. Hajime Ohgushi
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  5. Kunio Yonemasu
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Dohi, Y., Shimaoka, H., Ikeuchi, M. et al. Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture. Biol Trace Elem Res 104, 57–69 (2005). https://doi.org/10.1385/BTER:104:1:057

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  • DOI: https://doi.org/10.1385/BTER:104:1:057

Index Entries

  • Marrow mesenchymal stem cells
  • metallothionein isoforms
  • osteoblast differentiation
  • dexamethasone
  • alkaline phosphatase