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Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture

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Abstract

Effects of melatonin, extremely-low-frequency magnetic field (ELF-MF), and their combination on AT478 murine squamous cell carcinoma line were studied. Manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (Cu/ZnSOD), and glutathione peroxidase (GSH-Px) were used as markers of cells antioxidative status, and malondialdehyde (MDA) level was used as a marker of lipid peroxidation. After melatonin treatment, antioxidative enzyme activities were increased and MDA level was decreased. Application of ELF-MF on treated cells caused an increase of both superoxide dismutases activity and MDA level, but influence of ELF-MF on GSH-Px activity was negligible. All enzyme activity in culture medium containing melatonin (10−3, 10−4, 10−5 M) after exposure to ELF-MF were significantly diminished compared to cells treated only with melatonin. Also MDA levels after combined treatment with melatonin and ELF-MF were significantly decreased. Observed changes were statistically significant (p<0.05). These results strongly suggest that ELF-MF attenuates antioxidative actions of melatonin on cellular level.

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Authors and Affiliations

  1. Department of Physiology, Silesian University School of Medicine, Zabrze, Poland

    K. Żwirska-Korczala, M. Adamczyk-Sowa & P. Sowa

  2. Department of Biochemistry, Silesian University School of Medicine, Zabrze, Poland

    R. Polaniak & E. Birkner

  3. A. Chelkowski Institute of Physics, Department of Medical Physics, University of Silesia, Katowice, Poland

    Z. Drzazga

  4. Department of Physiology, Jagiellonian University School of Medicine, Cracow, Poland

    T. Brzozowski & S. J. Konturek

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  1. K. Żwirska-Korczala
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Żwirska-Korczala, K., Adamczyk-Sowa, M., Polaniak, R. et al. Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture. Biol Trace Elem Res 102, 227–243 (2004). https://doi.org/10.1385/BTER:102:1-3:227

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  • DOI: https://doi.org/10.1385/BTER:102:1-3:227

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