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Effect of nickel(II) chloride on iron content in rat organs after oral administration


The effect of oral administration of nickel(II) chloride on iron content in serum and certain body organs of rats was investigated. The male adult rats were given 300 and 1200 ppm Ni in drinking water for 90 d. The iron content in serum, liver, kidney, lung, spleen, and brain was analyzed 30 and 90 d postexposure. The hemoglobin, hematocrit, and body and organ weights were also measured. Nickel given in drinking water led to a pronounced increase in iron content in serum and the liver, as compared to control rats. This effect was related to Ni concentration in the water. There was not great time-dependent difference in the iron content as a response to continuous nickel treatment, except the lung of 1200-ppm Ni-treated rats. In relation to hematological parameters, Ni supplementation did not affect any of them. Body weight significantly decreased, and lung weight was significantly increased in 1200-ppm Ni-treated rats. The results of this study indicate that nickel ingestion (300 and 1200 ppm in the drinking water) induces the iron uptake by serum and some organs of rats. The highest amount of iron was found in the liver of all exposed animals, and the time-dependent difference in iron content was observed in the lung of 1200-ppm Ni-treated rats.

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  1. 1.

    M. Athar, S. K. Hasan, and R. C. Srivastava, Evidence for the involvement of hydroxyl radicals in nickel mediated enhancement of lipid peroxidation: implications for nickel carcinogenesis, Biochem. Biophys. Res. Commun. 147, 1276–1281 (1987).

    PubMed  Article  CAS  Google Scholar 

  2. 2.

    M. Misra, R. E. Rodriquez, and K. S. Kasprzak, Nickel induced lipid peroxidation in the rat: correlation with nickel effect on antioxidant defense systems, Toxicology 64, 1–17 (1990).

    PubMed  Article  CAS  Google Scholar 

  3. 3.

    Ch. Y. Chen, Y. L. Huang, and T. H. Lin, Lipid peroxidation in liver of mice administrated with nickel chloride. With special reference to trace elements and antioxidants. Biol. Trace Element Res. 61, 193–205 (1998).

    CAS  Google Scholar 

  4. 4.

    P. D. Whanger, Effects of dietary nickel on enzyme activities and mineral contents in rats, Toxicol. Appl. Pharmacol. 25, 323–331 (1973).

    PubMed  Article  CAS  Google Scholar 

  5. 5.

    B. Elsenhans, G. Schmolke, K. Kolb, J. Stokes, and W. Forth, Metal-metal interactions among dietary toxic and essential trace metals in the rat, Ecotoxicol. Environ. Safety 14, 275–287 (1987).

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    J. S. Oosting, A. G. Lemmens, G. J. Van Den Berg, and A. C. Beynen, Iron, copper, and zinc status in rats fed supplemental nickel, Biol. Trace Element Res. 31, 63–70 (1991).

    CAS  Article  Google Scholar 

  7. 7.

    J. Cartana, L. Arola, and A. Mas, Effects of acute nickel toxicity upon plasma and liver metal homeostasis as a function of sex, Toxicology 69, 133–141 (1991).

    PubMed  CAS  Google Scholar 

  8. 8.

    WHO, Environmental Health Criteria, Nickel, World Health Organization, Geneva, Vol. 108 (1991).

    Google Scholar 

  9. 9.

    M. Cempel and K. Janicka, Distribution of nickel, zinc and copper in rats organs after oral administration of nickel(II) chloride, Biol. Trace Element Res. 90, 215–226 (2002).

    Article  CAS  Google Scholar 

  10. 10.

    K. Janicka and M. Cempel, Lipid peroxidation and selected antioxidants in rat liver after oral exposure to nickel(II) chloride, Bromat. Chem. Toksykol. 34, 291–295 (2001).

    CAS  Google Scholar 

  11. 11.

    R. H. Thompson and W. J. Blacchflower, Wet-ashing apparatus to prepare biological materials for atomic absorption spectrophotometry, Lab. Pract. 20, 859–861 (1971).

    PubMed  CAS  Google Scholar 

  12. 12.

    M. Pinta, Absorpcyjna Spektrometria Atomowa. Zastosowania w Analizie Chemicznej, PWN, Warszawa (1977).

    Google Scholar 

  13. 13.

    M. P. Dieter, C. W. Jameson, A. N. Tucker, et al., Evaluation of tissue disposition, myelopoietic, and immunologic responses in mice after long-term exposure to nickel sulfate in the drinking water, J. Toxicol. Environ. Health 24, 357–372 (1988).

    PubMed  CAS  Article  Google Scholar 

  14. 14.

    J. A. Knight, M. R. Plowman, S. M. Hopfer, and F. W. Sunderman, Pathological reactions in lung, liver, thymus, and spleen of rats after subacute parenteral administration of nickel sulfate, Ann. Clin. Lab. Sci. 21, 275–283 (1991).

    PubMed  CAS  Google Scholar 

  15. 15.

    M. Comporti, Serial review: iron and cellular redox status, Free Radical Biol. Med. 32, 565–567 (2002).

    Article  CAS  Google Scholar 

  16. 16.

    S. J. Stohs and D. Bagchi, Oxidative machanisms in the toxicity of metal ions, Free Radical Biol. Med. 18, 321–336 (1995).

    Article  CAS  Google Scholar 

  17. 17.

    F. W. Sunderman, A. Marzouk, S. M. Hopfer, O. Zaharia, and M. C. Reid, Increased lipid peroxidation in tissues of nickel chloride-treated rats, Ann. Clin. Lab. Sci. 15, 229–236 (1985).

    PubMed  CAS  Google Scholar 

  18. 18.

    J. J. Clary, Nickel chloride-induced metabolic changes in the rat and guinea pig, Toxicol. Appl. Pharmacol. 31, 55–65 (1975).

    PubMed  Article  CAS  Google Scholar 

  19. 19.

    J. Tallkvist and H. Tjalve, Effect of dietary iron-deficiency on the disposition of nickel in rats, Toxicol. Lett. 92, 131–138 (1997).

    PubMed  Article  CAS  Google Scholar 

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Correspondence to Maria Cempel.

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Cempel, M. Effect of nickel(II) chloride on iron content in rat organs after oral administration. Biol Trace Elem Res 102, 189 (2004).

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Index Entries

  • Interaction
  • nickel chloride
  • nickel
  • iron
  • deposition
  • rat organs