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Validation of method for total selenium determination in yeast by flame atomic absorption spectrometry

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Abstract

A procedure using open digestion followed by flame atomic absorption spectrometry is described for measuring the total selenium content of Se-enriched yeast. The limits of detection and quantitation were 2.5 mg/L and 5 mg/L Se, respectively. The signal response was linear over the range of 5–50 mg/L Se, and the average recovery from spiked samples was 98.9%. The validated method was used to measure the Se content of Se-enriched yeast reference material and produced a result of 2145±38 mg/kg (n=3), which is in good agreement with the certified level of 2125 ±65 mg/kg.

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Authors and Affiliations

  1. Alltech Biotechnology Centre, Dunboyne, Co. Meath, Ireland

    Cathal D. Connolly & Ronan F. Power

  2. Department of Chemistry, National University of Ireland, Galway, Ireland

    Michael J. Hynes

Authors
  1. Cathal D. Connolly
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  2. Ronan F. Power
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  3. Michael J. Hynes
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Connolly, C.D., Power, R.F. & Hynes, M.J. Validation of method for total selenium determination in yeast by flame atomic absorption spectrometry. Biol Trace Elem Res 100, 87–93 (2004). https://doi.org/10.1385/BTER:100:1:087

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  • DOI: https://doi.org/10.1385/BTER:100:1:087

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