Abstract
Candida guilliermondii cells were immobilized in Ca-alginate beads and used for xylitol production from concentrated sugarcane bagasse hydrolysate. A full factorial design was employed to determine whether variations in the immobilization conditions would have any effects on the beads, chemical stability and on the xylitol production rates. Duplicate fermentation runs were carried out in 125-mL Erlenmeyer flasks maintained in a rotatory shaker at 30°C and 200 rpm for 72h. Samples were periodically analyzed to monitor xylose and acetic acid consumption, xylitol production, free cell growth, and bead solubilization. Concentrations of sodium alginate at 20.0 g/L and calcium chloride at 11.0 g/L and bead curing time of 24 h represented the most appropriate immobilization conditions within the range of conditions tested.
Similar content being viewed by others
References
Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1998), Bioresour. Technol. 65, 191–201.
Saska, M., Chen, F., and Vatelot, A. (1999), Int. Sugar J. 101, 354–358.
Winkelhausen, E. and Kuzmanova, S. (1998), J. Ferment. Bioeng. 86, 1–14.
Silva, S. S., Felipe, M. G. A., and Mancilha, I. M. (1998), Appl. Biochem. Biotechnol. 70/72, 331–339.
Felipe, M. G. A., Vitolo, M., Mancilha, I. M., and Silva, S. S. (1997), J. Ind. Microbiol. Biotechnol. 18, 251–254.
Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1998), Bioresour. Technol. 66, 25–40.
Parajó, J. C., Domínguez, H., and Domínguez, J. M. (1996), Biotechnol. Lett. 18, 593–598.
Carvalho, W., Silva, S. S., Vitolo, M., and Mancilha, I. M. (2000), Z. Naturforsch. 55c, 213–217.
Domínguez, J. M., Cruz, J. M., Roca, E., Domínguez, H. and Parajó, J. C. (1999), Appl. Biochem. Biotechnol. 81, 119–130.
Pilkington, P. H., Margaritis, A., Mensour, N. A., and Russel, I. (1998), J. Inst. Brew. 104, 19–31.
Jen, A. C., Wake, C., and Mikos, A. G. (1996), Biotechnol. Bioeng. 50, 357–364
Roca, E., Meinander, N., and Hahn-Hägerdal, B. (1996), Biotechnol. Bioeng. 51, 317–326.
Ogbonna, J. C., Amano, Y., and Nakamura, K. (1989), J. Ferment. Bioeng. 67, 92–96.
Alves, L. A., Felipe, M. G. A., Almeida e Silva, J. B., Silva, S. S., and Prata, A. M. R. (1998), Appl. Biochem. Biotechnol. 70/72, 89–98.
Barbosa, M. F. S., Medeiros, M. B., Mancilha, I. M., Schneider, H., and Lee, H. (1988), J. Ind. Microbiol. 3, 241–251.
Box, G. E. P., Hunter, W. G., and Hunter, J. S. (1978), Statistics for Experimenters, 1st ed., John Wiley & Sons, NY.
Chen, K. C. and Huang, C. T. (1989), Enzyme Microb. Technol. 10, 284–292.
Omar, S. H. (1993), Appl. Microbiol. Biotechnol. 40, 173–181.
Yahashi, Y., Hatsu, M., Horitsu, H., Kawai, K., Suzuki, T., and Takamizawa, K. (1996), Biotechnol. Lett. 18, 1395–1400.
Corcoran, E. (1985), in Topics in Enzyme and Fermentation Biotechnology, vol. 10, Wiseman, A., ed., Ellis Horwood, England, pp. 12–50.
Sene, L., Felipe, M. G. A., Vitolo, M., Silva, S. S., and Mancilha, I.M. (1998), J. Basic Microbiol. 38, 61–69.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Carvalho, W., Silva, S.S., Converti, A. et al. Use of immobilized Candida yeast cells for xylitol production from sugarcane bagasse hydrolysate. Appl Biochem Biotechnol 98, 489–496 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:489
Issue Date:
DOI: https://doi.org/10.1385/ABAB:98-100:1-9:489