Abstract
The yeast Candida rugosa produces multiple lipase isoenzymes sharing high sequence homology but with some differences in their catalytic properties. The regulation of C. rugosa lipase (CRL) synthesis and secretion in C. rugosa obeys a complex pattern. Fermentation processes for both wildtype and mutant C. rugosa strains are available for lipase production. Native CRL preparations have been extensively used for biotransformations. However, their inherent mixture of isoforms with variable profiles complicates interpretation and brings into question the reproducibility achieved between preparations. Although heterologous CRLs gene expression had been hampered owing to a nonuniversal codon usage, recent advances have made heterologous CRLs available. This will expand and improve the industrial utility of CRLs even further. The purpose of this review is to provide a summary of the recent advances on the production of native and recombinant lipases by C. rugosa.
Similar content being viewed by others
References
Jaeger, K. E., Ransac, S., Dijkstra, B. W., Colson, C., van Heuvel, M., and Misset, O. (1994), FEMS Microbiol. Rev. 15(1), 29–63.
Jaeger, K. E. and Reetz, M. T. (1998), Tibtech 16(9), 396–403.
Bailie, P., McNerlen, S., Robinson, E., and Murphy, W. (1994), Appl. Biocatal. 14, 11–13.
Bjorkling, F., Godtfredsen, S. E., and Kirk, O. (1991), Tibtech 9(10)P, 360–363.
Pandey, A., Benjamin, S., Soccol, C. R., Nigam, P., Krieger, N., and Soccol, V. T. (1999), Biotechnol. Appl. Biochem. 29(Pt. 2), 119–131.
Benjamin, S. and Pandey, A. (1998), Yeast 14, 1069–1087.
Schmidt-Dannert, C. (1999), Biorg. Med. Chem. 7(10), 2123–2130.
Rogalska, E., Douchert, I., and Verger, R. (1997), Biochem. Soc. Trans. 25(1), 161–164.
Ghosh, P. K., Saxena, R. K., Gupta, R., Yadav, R. P., and Davidson, S. (1996), Sci. Prog. 79(Pt. 2), 119–157.
Jaeger, K. E., Dijkstra, B. W., and Reetz, M. T. (1999), Annu. Rev. Microbiol. 53, 315–351.
Sarda, L. and Desnuelle, P. (1958), Biochim. Biophys. Acta 30, 513–521.
Ransac, S., Ivanova, M., Panaiotov, I., and Verger, R. (1999), Methods Mol. Biol. 109, 279–302.
Momsen, W. E. and Brockman, H. L. (1997), Methods Enzymol. 286, 292–305.
Labourdenne, S., Cagna, A., Delorme, B., Esposito, G., Verger, R., and Riviere, C. (1997), Methods Enzymol. 286, 306–326.
Verger, R. (1997), Tibtech 15(1), 32–38.
Kierkels, J. G. T., Vleugels, L. F. W., Gelade, E. T. F., Vermeulen, D. P., Kamphuis, J., Wandrey, C., and Vandentweel, W. J. J. (1994), Enzyme Microb. Technol. 16(6), 513–521.
Helistö, P. and Korpela, T. (1998), Enzyme Microb. Technol. 23(1–2), 113–117.
Rua, M. L., Díaz-Mauriño, T., Fernández, V. M., Otero, C., and Ballesteros, A. (1993), Biochim. Biophys. Acta 1156(2), 181–189.
Jensen, R. E. (1983), Lipids 18(9), 650–657.
Jaeger, K. E., Liebeton, K., Zonta, A., Schimossek, K., and Reetz, M. T. (1996), Appl. Microbiol. Biotechnol. 46(2), 99–105.
Thomson, C. A., Delaquis, P. J., and Mazza, G. (1999), Crit. Rev. Food Sci. Nutr. 39(2), 165–187.
Erdman, H., Vorderwülbecke, T., Schmid, R., and Kieslich, K. (1990), in Lipases: Structure, Mechanism and Genetic Engineering, vol. 16, Alberghina, L., Schmid, R. D., and Verger, R., eds., GBF monographys, VCH, Weinheim, Germany, pp. 425–428.
Rapp, P. and Backhaus, S. (1992), Enzyme Microb. Technol. 14(11), 938–943.
Arpigny, J. L. and Jaeger, K. E. (1999), Biochem. J. 343, 177–183.
White, J. S. and White, D. C. (1997), Source Book of Enzymes, CRC Press, New York.
Kawaguchi, Y. and Honda, H. (1990), in Lipases: Structure, Mechanism and Genetic Engineering, vol. 16, Alberghina, L., Schmid, R. D., and Verger, R., eds., GBF monographs, VCH, Weinheim, Germany, pp. 221–230.
Lotti, M., Grandori, R., Fusetti, F., Longhi, S., Brocca, S., Tramontano, A., and Alberghina, L. (1993), Gene 124(1), 45–55.
Lotti, M., Tramontano, A., Longhi, S., Fusetti, F., Brocca, S., Pizzi, E., and Alberghina, L. (1994), Protein Eng. 7(4), 531–535.
Lotti, M. and Alberghina, L. (1996), in Engineering of/with Lipases, Malcata, F. X., ed., Kluwer Academic, Dordrecht, The Netherlands, pp. 115–124.
Diczfalusy, M. A., Hellman, U., and Alexon, S. E. H. (1997), Arch. Biochem. Biophys. 348, 1–8.
Sánchez, A., Ferrer, P., Serrano, A., Pernas, M. A., Valero, F., Rúa, M. L., Casas, C., and Solà, C. (1999), Enzyme Microb. Technol. 25(3–5), 214–223.
Kaiser, R., Erman, M., Duax, W. L., Ghosh, D., and Jörnvall, H. (1994), FEBS Lett. 337(2), 123–127.
Lee, G. C., Tang, S. J., Sun, K. H., and Shaw, J. F. (1999), Appl. Environ. Microbiol. 65(9), 3888–3895.
Mileto, D., Brocca, S., Lotti, M., Takagi, M., Alquati, C., and Alberghina, L. (1998), Chem. Phys. Lipids 93, 47–55.
López, C., Guerra, N. P., and Rua, M. L. (2000), Biotechnol. Lett. 22, 1291–1294.
Iwai, M. and Tsujisaka, Y. (1994), Agric. Biol. Chem. 38, 1241–1247.
Patkar, S. A., Bjorkling, F., Zundel, M., Schulein, M., Svendsen, A., and Heldt-Hansen, H. P. (1993), Ind. J. Chem. 32(Pt. 1), 76–80.
Ohnishi, K., Yoshida, Y., and Sekiguchi, J. (1994), J. Ferment. Bioeng. 77(5), 490–495.
Catoni, E., Schmidt-Dannert, C., Brocca, S., and Schmid, R. D. (1997), Biotechnol. Tech. 11(9), 689–695.
Fu, Y., Ibrahim, A. S., Fonzi, W., Zhou, X., Ramos, C. F., and Ghannoum, M. A. (1997), Microbiology 143, 331–340.
Grochulski, P., Li, Y., Schrag, J., Bouthillier, F., Smith, P., Harrison, D., Rubin, B., and Cygler, M. (1993), J. Biol. Chem. 268(17), 12,843–12,847.
Ghosh, D., Wawrzak, Z., Pletnev, V. Z., Li, N., Kaiser, R., Pangborn, W., Jornvall, H., Erman, M., and Duax, W. L. (1995), Structure 3, 279–288.
Cygler, M. and Schrag, J. D. (1999), Biochim. Biophys. Acta 1441, 205–214.
Grochulski, P., Li, Y., Schrag, J. D., and Cygler, M. (1994), Protein Sci. 3(1), 82–91.
Brocca, S., Persson, M., Wehtje, E., Adlercreutz, P., Alberghina, L., and Lotti, M. (2000), Protein Sci. 9, 985–990.
Tang, S. J., Sun, K. H., Sun, G. H., Chang, T. Y., and Lee, G. C. (2000), Protein Express. Purif. 20, 308–313.
Lakshmi, B. S., Kangueane, B., Abraham, B., and Pennatur, G. (1999), Lett. Appl. Microbiol. 29(1), 66–70.
Gordillo, M. A., Obradors, N., Montesinos, J. L., Valero, F., Lafuente, J., and Solà, C. (1995), Appl. Microbiol. Biotechnol. 43(1), 38–41.
Valero, F., Ayats, F., López-Santín, J., and Poch, M. (1988), Biotechnol. Lett. 10(10), 741–744.
Tsujisaka, Y., Iwai, M., Fukumoto, J., and Okamoto, Y. (1973), Agric. Biol. Chem. 37(4), 837–842.
Valero, F., del Río, J. L., Poch, M., and Solà, C. (1991), J. Ferment. Bioeng. 72(5), 399–401.
Wang, Y., Luopa, J., Rajalahti, T., and Linko, S. (1995). Biotechnol. Tech. 9(10), 741–746.
Sokolovska, I., Albasi, C., Riba, J. P., and Bales, V. (1998), Bioprocess Eng. 19(3), 179–186.
Emery, A. N., Jan, D. C. H., and Al-Rubeai, M. (1995), Appl. Microbiol. Biotechnol. 43(6), 1028–1033.
Ota, Y., Miyairi, S., and Yamada, K. (1968), Agric. Biol. Chem. 32(12), 1476–1478.
Obradors, N., Montesinos, J. L., Valero, F., Lafuente, J., and Solà, C. (1993), Biotechnol. Lett. 15(4), 357–360.
Dalmau, E., Montesinos, J. L., Lotti, M., and Casas, C. (2000), Enzyme Microb. Technol. 26(9–10), 657–663.
Nahas, E. (1988), J. Gen. Microbiol. 134(Pt. 1), 227–233.
Baillargeon, M. W., Bistline, R. G., and Sonnet, P. E. (1989), Appl. Microbiol. Biotechnol. 30(1), 92–96.
Rapp, P. (1995), Enzyme Microb. Technol. 17(9), 832–838.
Lotti, M., Monticelli, S., Montesinos, J. L., Brocca, S., Valero, F., and Lafuente, J. (1998), Chem. Phys. Lipids 93, 143–148.
Chang, R. C., Chou, S. J., and Shaw, J. F. (1994), Biotechnol. Appl. Biochem. 19(Pt 1), 93–97.
Montesinos, J. L., Obradors, N., Gordillo, M. A., Valero, F., Lafuente, J., and Solà, C. (1996), Appl. Biochem. Biotechnol. 59(1), 25–37.
Gradova, N. B., Belov, A. P., and Guselnikova, T. V. (1990), Acta Biotechnol. 10(2), 169–177.
Gordillo, M. A., Sanz, A., Sánchez, A., Valero, F., Montesinos, J. L., Lafuente, J., and Solà, C. (1998), Biotechnol. Bioeng. 60(2), 156–168.
Benjamin, S. and Pandey, A. (1996), Bioresour. Technol. 55(2), 167–170.
Christakopoulos, P., Tzia, C., Kekos, D., and Macris, B. J. (1992), Appl. Microbiol. Biotechnol. 38(2), 194–197.
Salleh, A. B., Musani, R., Basri, M., Ampon, K., Yunus, W. M. Z., and Razac, C. N. A. (1993), Can. J. Microbiol. 39(10), 978–981.
Pokorny, D., Friedrich, J., and Cimerman, A. (1994), Biotechnol. Lett. 16(4), 363–366.
Papaparaskevas, D., Christakopoulos, P., Kekos, D., and Macris, B. J. (1992), Biotechnol. Lett. 14(5), 397–402.
Shimada, Y., Sugihara, A., Nagao, T., and Tominaga, Y. (1992), J. Ferment. Bioeng. 74(2), 77–80.
Linko, Y. Y. and Wu, X. Y. (1996), J. Chem. Technol. Biotechnol. 65(2), 163–170.
Sidebottom, C. M., Charton, E., Dunn, P. P., Mycock, G., Davies, C., Sutton, J. L., Macrae, A. R., and Slabas, A. R. (1991), Eur. J. Biochem. 202(2), 485–491.
Bertolini, M. C., Laramee, L., Thomas, D. Y., Cygler, M., Schrag, J. D., and Vernet, T. (1994), Eur. J. Biochem. 219(1–2), 119–125.
Pignède, G., Wang, H., Fudalej, F., Gaillardin, C., Seman, M., and Nicaud, J. M. (2000), J. Bacteriol. 182(10), 2802–2810.
Ratledge, C. and Evans, C. T. (1989), in The Yeast, vol. 3, Rose, A. H. and Harrison, J. H., eds., Academic, New York, pp. 372–385.
Dalmau, E. (1999), PhD thesis, Universitat Autònoma de Barcelona, Barcelona, Spain.
Eisai Co., Ltd. (1976), Japanese patent JP 76 57,889.
Mitsui Toatsu Chemicals Co., Ltd. (1970), Japanese patent JP 70 08,632.
Meito Sangyo Co. Ltd. (1964), Japanese patent JP 2987 (′64).
Amano-Pharm. (1990), Japanese patent JP J02086787.
Amano-Pharm. (1988), Japanese patent JP J63304992.
Meito-Sangyo Co., Ltd. (1987), Japanese patent JP J62019090.
Meito-Sangyo Co. Ltd. (1983), Japanese patent JP J58051889.
Mulchandani, A. and Bassi, A. S. (1995), Crit. Rev. Biotechnol. 15(2), 105–124.
Olsson L. and Nielsen, J. (1997), Tibtech 15(12), 517–522.
Valero, F., Poch, M., Solà, C., Santos Lapa, R. A., and Costa Lima, J. L. F. (1991), Biotechnol. Tech. 5(4), 251–254.
Cos, O., Montesinos, J. L., Lafuente, J., Solà, C., and Valero, F. (2000), Biotechnol. Lett. 22, 1783–1788.
Sánchez, A., Gordillo, M. A., Montesinos, J. L., Valero, F., and Lafuente, J. (1999), J. Biosci. Bioeng. 87(4), 500–506.
Chattaway, T., Demain, A. L., and Stephanopoulos, G. (1992), Biotechnol. Prog. 8(1), 81–84.
Heinzle, E. and Reuss, M. (1987), Mass Spectrometry in Biotechnological Process Analysis and Control, Plenum, New York.
Von Schalien, R., Fagervick, F., Saxén, B., Ringbom, K., and Rydström, M. (1995), Biotechnol. Bioeng. 48(6), 631–638.
Montesinos, J. L., Campmajó, C., Iza, J., Valero, F., Lafuente, J., and Solà, C. (1993), Biotechnol. Tech. 7(7), 429–434.
Montesinos, J. L., Campmajó, C., Iza, J., Valero, F., Lafuente, J., and Solà, C. (1994), Process Contr. Qual. 5, 237–244.
Cheruy, A. (1997), J. Biotechnol. 52(3), 193–199.
Stephanopoulos, G. and San, K. Y. (1984), Biotechnol. Bioeng. 26(10), 1176–1188.
Albiol, J., Campmajó, C., Casas, C., and Poch, M. (1993), Biotechnol. Prog. 11(1), 88–92.
Van der Heijden, R. T. J. M., Hellinga, C., Luyben, K. C. A. M., and Honderd, G. (1989), Tibtech 7(8), 205–209.
Cazzador, L. and Lubenova, V. (1995), Biotechnol. Bioeng. 47(6), 626–632.
Farza, M., Hammouri, H., Jallut, C., and Liéto, J. (1999), AIChE 45, 93–106.
Ljung, L. and Soderstrom, T. (1983), Theory and Practice of Recursive Identification, MIT Press, Cambridge, MA.
Chattaway, T. and Stephanopoulos G. (1989), Chem. Eng. Sci. 44(1), 41–48.
Thibault, V., Van Breusegem, V., and Cheruy, A. (1990), Biotechnol. Bioeng. 36(10), 1041–1048.
Montague, G. and Morris, A. J. (1994), Tibtech 12(8), 312–324.
Linko, S., Zhu, Y. H., and Linko, P. (1999), Tibtech 17(4), 155–162.
Valero, F., Lafuente, J., Poch, M., and Solà, C. (1990), Appl. Biochem. Biotechnol. 24–25, 591–602.
Montesinos, J. L., Lafuente, J., Gordillo, M. A., Valero, F., Solà, C., Charbonnier, S., and Cheruy, A. (1995), Biotechnol. Bioeng. 48(6), 573–584.
Aynsley, M., Hofland, A., Morris, A. J., Montague, G. A., and Di Massimo, C. (1993), Adv. Biochem. Eng. Biotechnol. 48, 1–27.
Simutis, R., Dors, M., and Lübbert, A. (1995), J. Biotechnol. 42(3), 285–290.
Tartakovsky, B., Ulitzur, S., and Sheintuch, M. (1995), Biotechnol. Prog. 11(1), 80–87.
Gomersall, R., Hitzmann, D., and Guthke, R. (1997), Bioprocess Eng. 17(2), 69–73.
Sargantanis, I. G. and Karim, M. N. (1998), Biotechnol. Bioeng. 60(1), 1–9.
Saucedo, V. M. and Karim, M. N. (1997), Biotechnol. Bioeng. 55(2), 317–327.
Ignatova, M., Lubenova, V., and Georgieva, P. (2000), Bioprocess Eng. 22(1), 79–84.
Del Río, J. L., Serra, P., Valero, F., Poch, M., and Solà, C. (1990), Biotechnol. Lett. 12(11), 835–838.
Serra, P., del Río, J. L., Robusté, J., Poch, M., Solà, C., and Cheruy, A. (1992), Bioprocess Eng. 8(3–4), 145–150.
Montesinos, J. L., Gordillo, M. A., Valero, F., Lafuente, J., Solà, C., and Valdman, B. (1997), J. Biotechnol. 52(3), 207–218.
Gordillo, M. A., Montesinos, J. L., Casas, C., Valero, F., Lafuente, J., and Solà, C. (1998), Chem. Phys. Lipids 93, 131–142.
Lee, S. Y. and Rhee, J. S. (1993), Enzyme Microb. Technol. 15(7), 617–623.
Rao, P. V., Jayaraman, K., and Lakshmanan, C. M. (1993), Process Biochem. 28(6), 385–389.
Rao, P. V., Jayaraman, K., and Lakshmanan, C. M. (1993), Process Biochem. 28(6), 391–395.
Benjamin, S. and Pandey, A. (1997), Acta Biotechnol. 17(3), 241–251.
Ferrer, P. and Solà, C. (1992), Appl. Microbiol. Biotechnol. 37(6), 737–741.
López, S., Valero, F., and Solà, C. (1996), Appl. Biochem. Biotechnol. 59(1), 15–24.
Benjamin, S. and Pandey, A. (1997), Process Biochem. 32(5), 437–440.
Dalmau, E., Sánchez, A., Montesinos, J. L., Valero, F., Lafuente, J., and Casas, C. (1998), J. Biotechnol. 59(3), 183–192.
Sánchez, A., Ferrer, P., Serrano, A., et al. (1999), J. Biotechnol. 69(2–3), 169–182.
Sánchez, A., De la Casa, R. M., Sinisterra, J. V., Valero, F., and Sánchez-Montero, J. M. (1999), Appl. Biochem. Biotechnol. 80(1), 65–75.
Weber, H. K., Stecher, H., and Faber, K. (1995), in Preparative Biotransformations, Robert, S. M., ed., Wiley, New York, pp. 5–21.
Barton, M. J., Hamman, J. P., Fichter, K. C., and Calton, C. J. (1990), Enzyme Microb. Technol. 12(8), 577–583.
Tsai, S. W. and Dordick, J. S. (1996), Biotechnol. Bioeng. 52(2), 296–300.
Lundell, K., Raijola, T., and Kanerva, L. T. (1998), Enzyme Microb. Technol. 22(2), 86–93.
Veeraragavan, K. and Gibbs, B. (1989), Biotechnol. Lett. 11(5), 345–348.
Brahimi-Horn, M. C., Guglielmino, M. L., Elling, L., and Sparrow, L. G. (1990), Biochim. Biophys. Acta 1042(1), 51–54.
Rothschild, N., Hadar, Y., and Dosoretz, C. (1997), Appl. Environ. Microbiol. 63(3), 857–861.
Wu, J. Y., Hanhove, J. L. K., Huang, Y. S., and Chen, Y. T. (1996), Biochem. Mol. Biol. Int. 39(4), 755–764.
Diczfalusy, M. A. and Alexson, S. E. H. (1996), Arch. Biochem. Biophys. 334, 104–112.
Cirigliano, M. C. and Carman, G. M. (1984), Appl. Environ. Microbiol. 48(4), 747–750.
Cirigliano, M. C. and Carman, G. M. (1985), Appl. Environ. Microbiol. 50(4), 846–850.
Chmiel, O., Traitler, H., Bauer, W., and Hammes, W. P. (1994), Food Biotechnol. 8(1), 7–33.
Chmiel, O., Traitler, H., Hammes, W. P., and Bauer, W. (1994), Food Biotechnol. 8(1), 35–56.
Stuer, W., Jaeger, K. E., and Winkler, U. K. (1986), J. Bacteriol. 168(3), 1070–1074.
Sabuquillo, P., Reina, J., Fernández, G., Guisán, J. M., and Fernández, R. (1998), Biochim. Biophys. Acta 1388(2), 337–348.
Bastida, A., Sabuquillo, P., Armisen, P., Fernández, R., Huguet, J., and Guisán, J. M. (1998), Biotechnol. Bioeng. 58(5), 486–493.
De la Casa, R. M., Sánchez-Montero, J. M., and Sinisterra, J. V. (1999), Biotechnol. Lett. 21(2), 123–128.
Rua, M. L. and Ballesteros, A. (1994), Biotechnol. Tech. 8(1), 21–26.
Kawaguchi, Y., Honda, H., Taniguchi-Morimura, J., and Iwasaki, S. (1989), Nature 341(6238), 164–166.
Fusetti, F., Brocca, S., Porro, D., and Lotti, M. (1996), Biotechnol. Lett. 18(3), 281–286.
Alberghina, L. and Lotti, M. (1996), in Engineering of/with Lipases, Malcata, F. X., ed., Kluwer Academic, Dordrecht, The Netherlands, pp. 219–228.
Brocca, S., Schmidt-Dannert, C., Lotti, M., Alberghina, L., and Schmid, R. D. (1998), Protein Sci. 7(6), 1415–1422.
Brocca, S., Schmidt-Dannert, C., Lotti, M., Alberghina, L., and Schmid, R. D. (1999), Patent WO9914338A1.
Brocca, S., Schmidt-Dannert, C., Lotti, M., Alberghina, L., and Schmid, R. D. (2000), Patent EP1012301A1.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ferrer, P., Montesinos, J.L., Valero, F. et al. Production of native and recombinant lipases by Candida rugosa . Appl Biochem Biotechnol 95, 221–255 (2001). https://doi.org/10.1385/ABAB:95:3:221
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:95:3:221