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Continuous cultivation of dilute-acid hydrolysates to ethanol by immobilized Saccharomyces cerevisiae

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Abstract

The continuous cultivation of immobilized Saccharomyces cerevisiae CBS 8066 on dilute-acid hydrolysates of forest residuals was investigated. The yeast cells were immobilized in 2–4% Ca-alginate beads. The 2% beads were not stable. However, the 3 and 4% beads were stable for at least 3 wk when an extra resource of calcium ions was available in the medium. The continuous cultivation of a dilute-acid hydrolysate by the immobilized cells at dilution rates of 0.3, 0.5, and 0.6 h−1 resulted in 86, 83, and 79% sugar consumption, respectively, and an ethanol yield between 0.45 and 0.48 g/g. The hydrolysate was fermentable at a dilution rate of 0.1 h−1 in a free-cell system but washed out at a dilution rate of 0.2 h−1. The continuous cultivation of a more inhibiting hydrolysate was not successful by either free- or immobilized-cell systems even at a low dilution rate of 0.07 h−1. However, when the hydrolysate was overlimed, it was fermentable by the immobilized cells at a dilution rate of 0.2 h−1.

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Authors and Affiliations

  1. Department of Chemical Reaction Engineering, Chalmers University of Technology, S-412 96, Göteborg, Sweden

    Mohammad J. Taherzadeh, Ria Millati & Claes Niklasson

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  1. Mohammad J. Taherzadeh
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  2. Ria Millati
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  3. Claes Niklasson
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Correspondence to Mohammad J. Taherzadeh.

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Taherzadeh, M.J., Millati, R. & Niklasson, C. Continuous cultivation of dilute-acid hydrolysates to ethanol by immobilized Saccharomyces cerevisiae . Appl Biochem Biotechnol 95, 45–57 (2001). https://doi.org/10.1385/ABAB:95:1:45

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  • DOI: https://doi.org/10.1385/ABAB:95:1:45

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