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New supports for enzyme immobilization based on copolymers of vinylene carbonate and acrylamide

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Abstract

In this study, a series of beadlike and hydrophilic supports containing reactive cyclic carbonate groups for enzyme immobilization were prepared via reverse-phase suspension copolymerization of the aqueous solutions of vinylene carbonate (VCA), acrylamide (AA), and N,N′-methylene bisacrylamide in paraffin oil. The supports were used as a matrix for immobilization of trypsin and showed a considerable capacity to couple with trypsin and reasonable retention of activity for the immobilized trypsin, depending on the immobilization conditions, such as the content of VCA structural units, reaction time, and pH of the medium.

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Author notes

  1. Lunhan Ding

    Present address: Department of Chemistry, The Ohio State University, 120 W. 18 Ave., Box 225, 43210-1106, Columbus, OH, USA

Authors and Affiliations

  1. Department of Chemistry, The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Nankai University, 300071, Tianjin, P. R. China

    Lunhan Ding, Yan Jiang, Lei Huang, Yonggang Li & Jiaxian Huang

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  1. Lunhan Ding
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  2. Yan Jiang
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  3. Lei Huang
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  4. Yonggang Li
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  5. Jiaxian Huang
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Correspondence to Lunhan Ding.

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Ding, L., Jiang, Y., Huang, L. et al. New supports for enzyme immobilization based on copolymers of vinylene carbonate and acrylamide. Appl Biochem Biotechnol 95, 11–21 (2001). https://doi.org/10.1385/ABAB:95:1:11

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  • DOI: https://doi.org/10.1385/ABAB:95:1:11

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