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Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from cottonseed oil and valeric acid in batch culture of Ralstonia sp. strain JC-64

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Abstract

A Ralstonia sp. strain JC-64 that is capable of accumulating poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P[3HB-co-3HV]) from cottonseed oil and valeric acid was isolated. By using a high limiting-nitrogen (HLN) mineral medium as the medium for the second stage of the fermentation process and by adding the two carbon sources at different times, a range of copolymers with 12–62 mol% of 3HV were produced from a series of HLN mineral mediums containing different compositions of cottonseed oil and valeric acid by Ralstonia sp. JC-64. The melting temperature (T m ) of polyhydroxybutyrate from cottonseed oil was 174°C and that of P(3HB-co-3HV) with the highest 3HV-mol fraction (62%) was 81°C.

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Authors and Affiliations

  1. Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama University, Gofuku, 930-8555, Toyama, Japan

    Cunjiang Song, Shin Ono, Choichiro Shimasaki & Masami Inoue

  2. The Pharmacy Education Center, 300400, Tianjin, China

    Lixing Zhao

Authors
  1. Cunjiang Song
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  2. Lixing Zhao
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  3. Shin Ono
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  4. Choichiro Shimasaki
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  5. Masami Inoue
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Correspondence to Masami Inoue.

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Song, C., Zhao, L., Ono, S. et al. Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from cottonseed oil and valeric acid in batch culture of Ralstonia sp. strain JC-64. Appl Biochem Biotechnol 94, 169–178 (2001). https://doi.org/10.1385/ABAB:94:2:169

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  • DOI: https://doi.org/10.1385/ABAB:94:2:169

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