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Characterization of bioconversion of fumarate to succinate by alginate immobilized Enterococcus faecalis RKY1

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Abstract

In this study, the immobilization characteristics of Enterococcus faecalis RKY1 for succinate production were examined. At first, three natural polymers—agar, κ-carrageenan, and sodium alginate—were tried as immobilizing matrices. Among these, sodium alginate was selected as the best gel for immobilization of E. faecalis RKY1. Efficient conditions for immobilization were established to be with a 2% (w/v) sodium alginate solution and 2-mm-diameter bead. The bioconversion characteristics of the immobilized cellsat various pH values and temperatures were examined and compared with those of free cells. The optimum pH and temperature of the immobilized cells were the same as for free cells, 7.0 and 38°C respectively, but the conversion ratio was higher by immobilization for all the other pH and temperature conditions tested. When the seed volume of the immobilized cells was adjusted to 10% (v/v), 30 g/L of fumarate was completely converted tosuccinate (0.973 g/g conversion ratio) after 12 h. In addition, the immobilized cells maintained a conversion ratio of >0.95 g/g during 4wk of storageat 4°C in a 2% (w/v) CaCl2 solution. In repetitive bioconversion experiments, the activity of the immobilized cells decreased linearly according to the number of times of reuse.

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Authors and Affiliations

  1. Department of Biochemical Engineering, Chonnam National University, 500-757, Kwangju, Korea

    Hwa-Won Ryu

  2. Department of Biomedical Engineering, Chonnam National University, 500-757, Kwangju, Korea

    Young-Jung Wee

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  1. Hwa-Won Ryu
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  2. Young-Jung Wee
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Correspondence to Hwa-Won Ryu.

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Ryu, HW., Wee, YJ. Characterization of bioconversion of fumarate to succinate by alginate immobilized Enterococcus faecalis RKY1. Appl Biochem Biotechnol 91, 525–535 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:525

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  • DOI: https://doi.org/10.1385/ABAB:91-93:1-9:525

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