Applied Biochemistry and Biotechnology

, Volume 91, Issue 1–9, pp 487–501 | Cite as

The effect of pretreatments on surfactin production from potato process effluent by Bacillus subtilis

  • David N. Thompson
  • Sandra L. Fox
  • Gregory A. Bala
Article

Abstract

Pretreatments of low-solids potato process effluent were tested for their potential to increase surfactin yield. Pretreatments included heat, removal of starch particulates, and acid hydrolysis. Elimination of contaminating vegetative cells was necessary for surfactin production. After autoclaving, 0.40 g/L of surfactin was produced from the effluent in 72 h, vs 0.24 g/L in the purified potato starch control. However, surfactin yields per carbon consumed were 76% lower from process effluent. Removal of starch particulates had little effect on the culture. Acid hydrolysis decreased growth and surfactant production, except 0.5 wt% acid, which increased the yield by 25% over untreated effluent.

Index Entries

Bacillus subtilis biosurfactant surfactin alternate feedstock enhanced oil recovery 

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References

  1. 1.
    Abdul, A. S., Gibson, T. L., and Rai, D. N. (1990), Ground Water 28, 920–926.CrossRefGoogle Scholar
  2. 2.
    Ang, C. C. and Abdul, A. S. (1991), Ground Water Monitor. Rev. 11, 121–127.CrossRefGoogle Scholar
  3. 3.
    Ellis, W. D., Payne, J. R., and McNab, G. D. (1985), EPA/600/S2-85/129, United States Environmental Protection Agency, Office of Research and Development, Washington, DC.Google Scholar
  4. 4.
    Rudolph, P. (1989), in Aquatic Toxicity Data Base, Federal Environmental Agency, Berlin.Google Scholar
  5. 5.
    Schröder, H. F. (1993), J. Chromatogr. 647, 219–234.CrossRefGoogle Scholar
  6. 6.
    Zajic, J. E. and Seffens, W. (1984), CRC Crit. Rev. Biotechnol. 1(2), 87–107.Google Scholar
  7. 7.
    Rosenberg, E. (1986), CRC Crit. Rev. Biotechnol. 3(3), 109–132.Google Scholar
  8. 8.
    Arima, K., Kakinuma, A., and Tamura, G. (1968), Biochem. Biophys. Res. Commun. 31, 488–494.CrossRefGoogle Scholar
  9. 9.
    Cooper, D. G., McDonald, C. R., Duff, S. J. B., and Kosaric, N. (1981), Appl. Environ. Microbiol. 42, 408–412.Google Scholar
  10. 10.
    Besson, F. and Michel, G. (1992), Biotechnol. Lett. 14(11), 1013–1018.CrossRefGoogle Scholar
  11. 11.
    Georgiou, G., Lin, S.-Y., and Sharma, M. M. (1992), Bio/Technology 10, 60–65.CrossRefGoogle Scholar
  12. 12.
    Lin, S.-Y. (1996), J. Chem. Technol. Biotechnol. 66, 109–120.CrossRefGoogle Scholar
  13. 13.
    Thompson, D. N., Fox, S. L., and Bala, G. A. (2000), Appl. Biochem. Biotechnol. 84–86, 917–930.CrossRefGoogle Scholar
  14. 14.
    Gherna, P. and Pienta, P., eds. (1989), in American Type Culture Collection Catalogue of Bacteria and Phages, 17th ed., American Type Culture Collection, Rockville, MD, p. 403.Google Scholar
  15. 15.
    Atlas, R. M. (1993), in Handbook of Microbiological Media, Parks, L. C., ed., CRC Press, Boca Raton, FL, p. 672.Google Scholar
  16. 16.
    Gerhardt, P., Murray, R. G. E., Wood, W. A., and Krieg, N. R., eds. (1994), in Methods for General and Molecular Bacteriology, American Society for Microbiology, Washington, DC, pp. 518, 519.Google Scholar
  17. 17.
    Bailey, J. E. and Ollis, D. F. (1986), in Biochemical Engineering Fundamentals, 2nd ed., Verina, K. and Martin, C. C., eds., McGraw-Hill, New York, p. 5.Google Scholar
  18. 18.
    Herd, M. D., Lassahn, G. D., Thomas, C. P., Bala, G. A., and Eastman, S. L., (1992), in Proceedings of the DOE Eighth Symposium on Enhanced Oil Recovery, SPE/DOE24206, Tulsa, OK.Google Scholar
  19. 19.
    Gerson, D. F. and Zajic, J. E. (1979), Process Biochem. 14, 20–29.Google Scholar
  20. 20.
    Sheppard, J. D. and Mulligan, C. N. (1987), Appl. Microbiol. Biotechnol. 27, 110–116.CrossRefGoogle Scholar
  21. 21.
    Grethlein, H. E. (1978), Biotechnol. Bioeng. 20, 503–525.CrossRefGoogle Scholar
  22. 22.
    Marsden, L. M. and Gray, P. P. (1986), CRC Crit. Rev. Biotechnol. 3(3), 235–276.CrossRefGoogle Scholar
  23. 23.
    Lee, J. (1997), J. Biotechnol. 56, 1–24.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc 2001

Authors and Affiliations

  • David N. Thompson
    • 1
  • Sandra L. Fox
    • 1
  • Gregory A. Bala
    • 1
  1. 1.Biotechnology DepartmentIdaho National Engineering and Environmental LaboratoryIdaho Falls

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