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Kinetics of ethanol fermentation with high biomass concentration considering the effect of temperature

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Abstract

A model of ethanol fermentation considering the effect of temperature was developed and validated. Experiments were performed in a temperature range from 28 to 40°C in continuous mode with total cell recycling using a tangential microfiltration system. The developed model considered substrate, product and biomass inhibition, as well as an active cell phase (viable) and an inactive (dead) phase. The kinetic parameters were described as functions of temperature.

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Abbreviations

D :

F/V=dilution rate (h−1)

F :

substrate feed flow rate (m3/h)

K dP :

coefficient of death by ethanol (m3/kg)

K dT :

coefficient of death by temperature (h−1)

K i :

substrate inhibition coefficient (m3/kg)

K s :

substrate saturation constant (kg/m3)

m :

constant in Eq. 5

m p :

ethanol production associated with growth (kg/[kg·h])

m x :

maintenance coefficient (kg/[kg·h])

n :

constant in Eq. 5

P :

product concentration (kg/m3)

P max :

product concentration when cell growth ceases (kg/m3)

r d :

kinetic rate of death (kg/[m3·h])

r p :

kinetic rate of ethanol formation (kg/[m3·h])

r s :

kinetic rate of substrate consumption (kg/[m3·h])

r x :

kinetic rate of growth (kg/[m3·h])

S :

substrate concentration (kg/m3)

S r :

feed substrate concentration (kg/m3)

V :

reactor volume (m3)

X d :

dead biomass concentration (kg/m3)

X max :

biomass concentration when cell growth ceases (kg/m3)

X f=Xv+Xd :

total biomass concentration (kg/m3)

X v :

viable biomass concentration (kg/m3)

Y μx :

yield of product based on cell growth (kg/kg)

Y s :

limit cellular yield (kg/kg)

γ:

ratio of concentration of intracellular to extracellular ethanol

μmax :

maximum growth rate (h−1)

σ:

ratio of dry cell weight perwet cell volume (kg/m3)

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Authors and Affiliations

  1. DEA/FEA/UNICAMP, Cx. Postal 6121, 1308 1-970, Campinas, SP, Brazil

    Daniel I. P. Atala & Francisco Maugeri

  2. DPQ/FEQ/UNICAMP, Cx. Postal 6066, 1308 1-970, Campinas, SP, Brazil

    Aline C. Costa & Rubens Maciel

Authors
  1. Daniel I. P. Atala
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  2. Aline C. Costa
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  3. Rubens Maciel
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  4. Francisco Maugeri
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Correspondence to Aline C. Costa.

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Atala, D.I.P., Costa, A.C., Maciel, R. et al. Kinetics of ethanol fermentation with high biomass concentration considering the effect of temperature. Appl Biochem Biotechnol 91, 353–365 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:353

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  • DOI: https://doi.org/10.1385/ABAB:91-93:1-9:353

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