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Enhancement of the conversion of toluene by Pseudomonas putida F-1 using organic cosolvents

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Abstract

Pseudomonas putida F-1 (ATCC700007) was used as a model organism in stirred tank reactors, to study conversion enhancement of poorly soluble substrates by organic cosolvents. After a literature study, silicone oil was used as a solvent system to enhance the mass transfer rate. To study the benefits of the organic solvent addition, batch experiments were conducted in two side-by-side fermentation vessels (experimental and control) at three different levels of silicone oil (10, 30, and 50%). Results showed that the presence of silicone oil resulted in a 100% increase in the toluene mass transfer compared to the control. Experiments in continuous stirred-tank reactors showed that improved conversion could beobtained, at higher agitation rates.

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Authors and Affiliations

  1. Bioprocessing Research and Development Center, Oak Ridge National Laboratory, PO Box 2008-6226, 37831-6226, Oak Ridge, TN

    Miguel Rodriguez, K. Thomas Klasson & Brian H. Davison

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  1. Miguel Rodriguez
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  2. K. Thomas Klasson
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  3. Brian H. Davison
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Correspondence to K. Thomas Klasson.

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Rodriguez, M., Thomas Klasson, K. & Davison, B.H. Enhancement of the conversion of toluene by Pseudomonas putida F-1 using organic cosolvents. Appl Biochem Biotechnol 91, 195–204 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:195

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  • DOI: https://doi.org/10.1385/ABAB:91-93:1-9:195

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