Abstract
We investigated the relationship between the synthesis of bacterial magnetic particles (BMPs) and the transcription of magA gene-encoding iron transport protein using synchronous culture of Magnetospirillum magneticum AMB-1. Synchronously cultured cells were subjected to transmission electron microscopic observation and fluorescence in situ hybridization. The average number of BMPs slowly increased in the cell with increasing cell size. A sharp increase in BMPs occurred just before cell division and resulted in maximum BMP production of 30 particles/cell. The transcription of magA was regulated immed iately before and after cell division
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References
Blakemore, R. P. (1982), Annu. Rev. Microbiol. 36, 217–238.
Balkwill, D. L., Maratea, D., and Blakemore, R. P. (1980), J. Bacteriol. 141, 1399–1480.
Matsunaga, T., Sakaguchi, T., and Tadokoro, F. (1991), Appl. Microbiol. Biotechnol. 35, 651–655.
Matsunaga, T., Kawasaki, M., Yu, X., Tsujimura, N., and Nakamura, N. (1996), Anal. Chem. 68, 3551–3554.
Nakamura, N., Hashimoto, K., and Matsunaga, T. (1991), Anal. Chem. 63, 268–272.
Nakamura, C., Burgess, J. G., and Matsunaga, T. (1995), J. Biol. Chem. 270, 28,392–28,396.
Nakamura, C., Kikuchi, T., Burgess, J. G., and Matsunaga, T. (1995), J. Biochem. 118, 23–27.
Matsunaga, T., Sato, R., Kamiya, S., Tanaka, T., and Takeyama, H. (1999), J. Magn. Magn. Mater. 194, 126–131.
Tanaka, T. and Matsunaga, T. (2000), Anal. Chem. 72, 3518–3522.
Matsunaga, T., Tsujimura, N., and Kamiya, S. (1996), Biotechnol. Techniques 10, 495–500.
Matsunaga, T., Togo, H., Kikuchi, T., and Tanaka, T. Biotechnol. Bioeng., in press.
Uchiyama, K. and Shioya, S. (1999), J. Biotechnol. 71, 133–141.
Mitsui, A., Kumazawa, S., Takahashi, A., Ikemoto, H., Cao, S., and Arai, T. (1986), Nature 323, 720–722.
Campbell, C., Takeyama, H., and Mitsui, A. (1994), J. Mar. Biotechnol. 2, 39–43.
Sato, R., Miyagi, T., Kamiya, S., Sagaguchi, T., Thornhill, R. H., and Matsunaga, T. (1995), FEMS Microbiol. Lett. 128, 15–20.
Blakemore, R. P., Maratea, D., and Wolfe, S. (1979), J. Bacteriol. 140, 720–729.
Chomczynski, P. (1987), in Current Protocols in Molecular Biology, vol. 3, Ausuble, F. M., ed., Wiley, New York, pp. 14.1.1–14.7.11.
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Yang, CD., Takeyama, H., Tanaka, T. et al. Synthesis of bacterial magnetic particles during cell cycle of Magnetospirillum magneticum AMB-1. Appl Biochem Biotechnol 91, 155–160 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:155
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DOI: https://doi.org/10.1385/ABAB:91-93:1-9:155