Abstract
Magnetic alginate beads were used to purify α-amylases from porcine pancreas, starchzyme, BAN 240L (a commercial purification from Bacillus subtilis), and wheat germ. The beads bound a significant level of α-amylase activity from porcine pancreas, BAN 240L, and wheat germ. In each case, the enzyme activity could be eluted by using 1.0 M maltose, a known competitive inhibitor of α-amylase. In the case of BAN 240L, 3.6-fold purification with 72% recovery of activity was observed. In the case of wheat germ enzyme, starting from the crude extract, 48-fold purification with 70% activity recovery was observed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis also indicated considerable purification in the latter case.
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Teotia, S., Gupta, M.N. Purification of α-amylases using magnetic alginate beads. Appl Biochem Biotechnol 90, 211–220 (2001). https://doi.org/10.1385/ABAB:90:3:211
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DOI: https://doi.org/10.1385/ABAB:90:3:211