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An improved method for extraction of β-carotene from blakeslea trispora

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Abstract

An improved method for the extraction of β-carotene from Blakeslea trispora is described. The fermentation broth was steamed at 121°C for 15 min, and the liquid was centrifuged at 5000g for 20 min. β-Carotene was removed from the biomass by extraction with absolute ethanol at a ratio of 1:100 at 30°C for 2 h in a rotary shaker incubator at 300 rpm. The carotenoid pigment was completely removed from the cells after three repeated extractions. The removal of β-carotene from B. trispora was higher during the first stage (75%) whereas in the other stages it was very slow.

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Authors and Affiliations

  1. Department of Food Science and Technology, Aristotle University of Thessaloniki, Box 250, 540 06, Thessaloniki, Greece

    T. Roukas & F. Mantzouridou

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  1. T. Roukas
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  2. F. Mantzouridou
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Correspondence to T. Roukas.

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Roukas, T., Mantzouridou, F. An improved method for extraction of β-carotene from blakeslea trispora . Appl Biochem Biotechnol 90, 37–45 (2001). https://doi.org/10.1385/ABAB:90:1:37

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  • DOI: https://doi.org/10.1385/ABAB:90:1:37

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