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Study of biocatalyst to produce ethanol from starch

Coimmobilization of glucoamylase and yeast in gel

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Abstract

This article presents a detailed study on the conditions for achieving a stable biocatalyst to be used in the production of ethanol from starch. Different pellets were used depending on which characteristic of the biocatalyst was being studied: (a) Saccharomyces cerevisiae entrapped in pectin or calcium alginate gel particles; (b) silica containing immobilized glucoamylase entrapped in pectin gel particles; or (c) pectin gel particles, with the silicaenzyme derivative and yeast coimmobilized. The influence of several variables on the mechanical resistance of the particle, on the viability of the microorganism, and on the rate of substrate hydrolysis was studied with biocatalyst. The best conditions found were 6% pectin gel, 2-mm particle diameter, and curein 0.2 M CaCl2·2H2O/60 mM acetate buffer, pH 4.2, for gel preparation; and 6.0 g/L of CaCl2·2H2O in the fermentation medium. Biocatalyst (c) was successfully tested for the production of ethanol from liquefield manioc flour syrup.

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Authors and Affiliations

  1. Departamento de Engenharia Química, Universidade Federal de São Carlos, C.P. 676, 13.565-905, São Carlos, SP, Brazil

    Raquel L. C. Giordano & Paulo C. Hirano

  2. Departamento de Engenharia Química, Universidade Federal do Ceará, Pici Campus, Bloco 710, Sala 32, Fortaleza, CE, Brazil

    Luciana R. B. Gonçalves

  3. Departamento de Engenharia Química, Universidade de São Paulo, São Paulo, SP, Brazil

    Willibaldo Schmidell Netto

Authors
  1. Raquel L. C. Giordano
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  2. Paulo C. Hirano
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  3. Luciana R. B. Gonçalves
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  4. Willibaldo Schmidell Netto
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Corresponding author

Correspondence to Raquel L. C. Giordano.

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Giordano, R.L.C., Hirano, P.C., Gonçalves, L.R.B. et al. Study of biocatalyst to produce ethanol from starch. Appl Biochem Biotechnol 84, 643–654 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:643

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:643

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