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Floating cultivation of marine cyanobacteria using coal fly ash

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Abstract

The aim of this study was to develop improved methodologies for bulk culturing of biotechnologically useful marine cyanobacteria in the open ocean. We have investigated the viability of using coal fly ash (CFA) blocks as the support medium in a novel floating culture system for marine microalgae. The marine cyanobacterium Synechococcus sp. NKBG 040607 was found to adhere to floating CFA blocks in liquid culture medium. Maximum density of attached cells of 2.0×108 cells/cm2 was achieved using sea water. The marine cyanobacterium Synechococcus sp. NKBG 042902 weaklyadhered to floating CFA blocks in BG-11 medium. Increasing the concentration of calcium ion in the culture medium enhanced adherence to CFA blocks.

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References

  1. Benemann, J. R., Tillet, D. M., and Weissaman, J. C. (1987), Trends. Biotechnol. 5, 47–53.

    Article  CAS  Google Scholar 

  2. Burgess, J. G., Iwamoto, K., Miura, Y., Takano, H., and Matsunaga, T. (1993), Appl. Microbiol. Biotechnol. 39, 456–459.

    Article  CAS  Google Scholar 

  3. Matsunaga, T., Burgess, J. G., Yamada, N., Komatsu, K., Yoshida, S., and Wachi, Y. (1993), Appl. Microbiol. Biotechnol. 39, 250–253.

    CAS  Google Scholar 

  4. Takano, H., Takeyama, H., Nakamura, N., Sode, K., Burgess, J. G., Manabe, E., Hirano, M., and Matsunaga, T. (1992), Appl. Biochem. Biotechnol. 34/35, 449–458.

    Article  Google Scholar 

  5. Takano, H., Takei, R., Manabe, H., Burgess, J. G., Hirano, M., and Matsunaga, T. (1995), Appl. Microbiol. Biotechnol. 43, 460–465.

    Article  CAS  Google Scholar 

  6. Miura, Y., Sode, K., Nakanura, N., Matsunaga, N., and Matsunaga, T. (1993), FEMS Microbiol. Lett. 107, 163–168.

    CAS  Google Scholar 

  7. Wake, H., Akasaka, A., Umetsu, H., Ozeki, Y., Shimomura, K., and Matsunaga, T. (1992), Appl. Microbiol. Biotechnol. 36, 684–688.

    Article  Google Scholar 

  8. Matsunaga, T., Takeyama, H., and Takano, H. (1999), The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation, John Wiley & Sons, New York.

    Google Scholar 

  9. Scott, C., Fletcher, R. L., and Bremer, G. B. (1996), Biofouling 10, 161–173.

    Article  CAS  Google Scholar 

  10. Wastman, B. A., Lind, J., Wetherbee, R., and Gretz, M. R. (1998), Plant. Physiol. 116, 1431–1441.

    Article  Google Scholar 

  11. Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., and Stanier, R. Y. (1979), J. Gen. Microbiol. 111, 1–61.

    Google Scholar 

  12. Eppley, R. W., Holmes, R. W., and Strickland, J. D. H. (1967), J. Exp. Mar. Biol. Ecol. 1, 191–208.

    Article  Google Scholar 

  13. Sudo, H., Yamada, A., Kokatsu, K., Nakamura, N., and Matsunaga, T. (1997), Appl. Microbiol. Biotechnol. 47, 78–82.

    Article  CAS  Google Scholar 

  14. Ayoub, G. M., Lee, Sang, I. L. L., and Koopman, B. (1986), Water Res. 20, 1265–1271.

    Article  CAS  Google Scholar 

  15. Neilan, B. A., Dittmann, E., Rouhiainen, L., Bass, R. A., Schaub, V., Sivonen, K., and Borner, T. (1999), J. Bacteriol. 181, 4089–4097.

    CAS  Google Scholar 

  16. Tsuji, K., Watanuki, T., Kondo, F., Watanabe, M. F., Nakazawa, H., Suzuki, M., Uchida, H., and Harada, K. (1997), Toxicon 35, 1033–1041.

    Article  CAS  Google Scholar 

  17. Rapala, J., Sivonen, K., Lyra, C., and Niemela, S. I. (1997), Appl. Environ. Microbiol. 63, 2206–2212.

    CAS  Google Scholar 

  18. Matsunaga, T., Takeyama, H., Sudo, H., Oyama, N., Ariura, S., Takano, H., Hirano, M., Burgess, J. G., Sode, K., and Nakamura, N. (1991), Appl. Biochem. Biotechnol. 28/29, 157–167.

    Google Scholar 

  19. Matsunaga, T., Nakamura, N., Tsuzuki, N., and Takeda, H. (1988), Appl. Microbiol. Biotechnol. 28, 373–376.

    Article  CAS  Google Scholar 

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Correspondence to Tadashi Matsunaga.

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Matsumoto, M., Yoshida, E., Takeyama, H. et al. Floating cultivation of marine cyanobacteria using coal fly ash. Appl Biochem Biotechnol 84, 51–57 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:51

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:51

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