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Two-dimensional analysis of proteins specific to the bacterial magnetic particle membrane from Magnetospirillum sp. AMB-1

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Abstract

We report the identification of five proteins expressed specifically on the bacterial magnetic particle (BMP) membrane of Magnetospirillum sp. AMB-1. These proteins are major components of the BMP membrane. The molecular weights were determined to be 12.0, 16.0, 24.8, 35.6, and 66.2 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Of these five, the 16.0-kDa protein was the most abundant in the BMP membrane. Furthermore, the 16.0-kDa protein consisted of two components each of differing pI. The 35.6-kDa protein was the second most abundant protein of the five detected.

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References

  1. Nakamura, C., Burgess, J. G., Sode, K., and Matsunaga, T. (1995), J. Biol. Chem. 270, 28,392–28,396.

    Article  CAS  Google Scholar 

  2. Nakamura, C., Kikuchi, T., Burgess, J. G., and Matsunaga, T. (1995), J. Biochem. 118, 23–27.

    CAS  Google Scholar 

  3. Frankel, R. B., Papaefthymiou, G. C., Blakemore, R. P., and O'Brien, W. (1983), Biochim. Biophys. Acta 763, 147–159.

    Article  CAS  Google Scholar 

  4. Bazylinski, D. A., Frankel, R. B., Heywood, B., Mann, S., King, J. W., Donaghay, P. L., and Hanson, A. K. (1995), Appl. Environ. Microbiol. 61, 3232–3239.

    CAS  Google Scholar 

  5. Gorby, Y. A., Beveridge, T. J., and Blakemore, R. P. (1988), J. Bacteriol. 170, 834–841.

    CAS  Google Scholar 

  6. Matsunaga, T., Sato, R., Kamiya, S., Tanaka, T., and Takeyama, H. (1999), J. Magn. Magn. Mater. 194, 126–131.

    Article  CAS  Google Scholar 

  7. Blakemore, R. P., Maratea, D., and Wolfe, R. S. (1979), J. Bacteriol. 140, 720–729.

    CAS  Google Scholar 

  8. Laemmli, U. K. (1970), Nature 227, 680–685.

    Article  CAS  Google Scholar 

  9. Li, S. J. and Cronan, J. E. Jr. (1992), J. Biol. Chem. 267, 16,841–16,847.

    CAS  Google Scholar 

  10. Okuda, Y., Denda, K., and Fukumori, Y. (1996), Gene 171, 99–102.

    Article  CAS  Google Scholar 

  11. Goebl, M. and Yanagida, M. (1991), Trends Biochem. Sci. 16, 173–177.

    Article  CAS  Google Scholar 

  12. Sikorski, R. S., Boguski, M. S., Goebl, M., and Hieter, P. (1990), Cell 60, 307–317.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, 184-8588, Tokyo, Japan

    Yoshiko Okamura, Haruko Takeyama & Tadashi Matsunaga

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  1. Yoshiko Okamura
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  2. Haruko Takeyama
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  3. Tadashi Matsunaga
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Correspondence to Tadashi Matsunaga.

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Okamura, Y., Takeyama, H. & Matsunaga, T. Two-dimensional analysis of proteins specific to the bacterial magnetic particle membrane from Magnetospirillum sp. AMB-1. Appl Biochem Biotechnol 84, 441–446 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:441

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:441

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