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Optimizing ammonia pressurization/depressurization processing conditions to enhance enzymatic susceptibility of dwarf elephant grass

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Abstract

An ammonia pressurization/depressurization process was investigated to evaluate the potential of producing reducing sugars from dwarf elephant grass, a warm-season forage. Moisture, temperature, and ammonia loading affected sugar yield (p<0.0001). At optimal conditions, ammonia processing solubilized 50.9% of the hemicellulose and raised the sugar yield (percentage of theoretical) from 18 to 83%. Glucose and xylose production were increased 3.2-and 8.2-fold, respectively. The mild processing conditions of the ammonia treatment (90–100°C, 5 min), the low enzyme loading (2 international filter paper units/g), and the short hydrolysis time (24 h), greatly enhance the potential of using for ages to produce sugars valuable for several applications.

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Authors and Affiliations

  1. Food Laboratory, Department of Chemistry, Science Faculty, University of Zulia, Grano de Oro Módulo, Maracaibo, Venezuela

    Alexis Ferrer & Betzabé Sulbarán-de-Ferrer

  2. Department of Animal Science, Texas A&M University, 101 Kleberg Center, 77843-2472, College Station, TX

    Floyd M. Byers

  3. Department of Chemical Engineering, Michigan State University, A 202 Engineering Building, 48824-1226, East Lansing, MI

    Bruce E. Dale

  4. Engineering Faculty, University of Zulia, Av. Goajira, Maracaibo, Venezuela

    Cateryna Aiello

Authors
  1. Alexis Ferrer
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  2. Floyd M. Byers
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  3. Betzabé Sulbarán-de-Ferrer
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  4. Bruce E. Dale
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  5. Cateryna Aiello
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Correspondence to Alexis Ferrer.

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Ferrer, A., Byers, F.M., Sulbarán-de-Ferrer, B. et al. Optimizing ammonia pressurization/depressurization processing conditions to enhance enzymatic susceptibility of dwarf elephant grass. Appl Biochem Biotechnol 84, 163–179 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:163

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:163

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