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Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation

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Abstract

Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compounds were analyzed in detail. Of all species tested the red alga Pachymeniopsis lanceolata contained the highest EPA concentration, and it was present as both the free and bound forms. EPA constituted 38.7% of total fatty acids, and polar lipids were the main constituent of the total lipids in P. lanceolata. EPA was obtained from the marine algae P. lanceolata by enzymatic hydrolysis of the total lipids extract using phospholipase A2(PLA2). The release of EPA reached a plateau after 10 min of enzymatic treatment. These results suggest that P. lanceolata is a useful natural source of EPA and that PLA2 treatment is a convenient method for obtaining EPA from the red alga.

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References

  1. Thorngren, M. and Gustafson, A. (1981), Lancet 2, 1190–1193.

    Article  PubMed  CAS  Google Scholar 

  2. Croset, M., Vericel, E., Rigaud, M., Hanss, M., Courpron, P., Dechavanne, M., and Lagarde, M. (1990), Thromb. Res. 57, 1–12.

    Article  PubMed  CAS  Google Scholar 

  3. Clubb, F. J., Jr., Schmitz, J. M., Butler, M. M., Buja, L. M., Willerson, J. T., and Campbell, W. B. (1989), Arteriosclerosis 9, 529–537.

    PubMed  CAS  Google Scholar 

  4. Schmidt, E. B., Varming, K., Svaneborg, N., and Dyerberg, J. (1992), Ann. Nutr. Metab. 36, 283–287.

    Article  PubMed  CAS  Google Scholar 

  5. Urakaze, M., Hamazaki, T., Soda, Y., Miyamoto, M., Ibuki, F., Yano, S., and Kumagai, A. (1986), Thromb. Res. 44, 673–682.

    Article  PubMed  CAS  Google Scholar 

  6. Wojenski, C. M., Silver, M. J., and Walker, J. (1991), Biochim. Biophys. Acta 1081, 33–38.

    PubMed  CAS  Google Scholar 

  7. Tamura, Y., Hirai, A., Terano, T., Yoshida, S. Takenaga, M., and Kitagawa, H. (1987), Jpn. Circ. J. 51, 471–477.

    PubMed  CAS  Google Scholar 

  8. Tsuruta, K., Ogawa, H., Yasue, H., Sakamoto, T., Miyao, Y., Tanae, H., and Kaiga, K. (1996), Coron. Artery Dis. 7, 837–842.

    Article  PubMed  CAS  Google Scholar 

  9. Shinozaki, K., Kambayashi, J., Kawasaki, T., Uemura, Y., Sakon, M., Shiba, E., Shibuya, T., Nakamura, T., and Mori, T. (1996), J. Atheroscler. Thromb. 2, 107–109.

    PubMed  CAS  Google Scholar 

  10. Lopez, A. D., Molina, G. E., Sanchez, P. J. A., Garcia, S. J. L., and Garcia, C. F. (1992), Aquaculture 102, 363–371.

    Article  Google Scholar 

  11. Yongmanitchai, W. and Ward, O. P. (1991), Phytochemistry 30, 2963–2968.

    Article  CAS  Google Scholar 

  12. Cohen, Z. and Cohen, S. (1991), J. Am. Oil Chem. Soc. 68, 16–19.

    CAS  Google Scholar 

  13. Sanchez, P. J. A. (1994), J. Appl. Phycol. 6, 475–478.

    Article  Google Scholar 

  14. Yongmanitchai, W. and Ward, O. P. (1991), Appl. Environ. Microbiol. 57, 419–425.

    PubMed  CAS  Google Scholar 

  15. Ohta, S., Chang, T., Aozasa, O., Kondo, M., and Miyata, H. (1992), J. Ferm. Bioeng. 74, 398–402.

    Article  CAS  Google Scholar 

  16. Dembitsky, V. M., Pechenkina, S. E. E., and Rozentsvet, O. A. (1991), Phytochemistry 30, 2279–2284.

    Article  CAS  Google Scholar 

  17. Fleurence, J., Gutbier, G., Mabeau, S., and Leray, C. (1994), J. Appl. Phycol. 6, 527–532.

    Article  CAS  Google Scholar 

  18. Hatakeda, K., Ito, S., Sato, O., Saito, N., Ikushima, Y., and Goto, T. (1993), The Chem. Soc. Jpn. 4, 408–409.

    Google Scholar 

  19. Folch, J., Lees, M., and Sloan-Stanley, G. H. (1957), J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  20. Shuster, C. Y., Froines, J. R., and Olcott, H. S. (1964), J. Am. Oil Chem. Soc. 41, 36–41.

    CAS  Google Scholar 

  21. Ryu, E. K. and MacCoss, M. (1979), J. Lipid Res. 20, 561–563.

    PubMed  CAS  Google Scholar 

  22. Norman, H. A. and Thompson, G. A. Jr. (1985), Arch. Biochem. Biophys. 242, 168–175.

    Article  PubMed  CAS  Google Scholar 

  23. Springer, M., Franke, H., and Pulz, O. (1994), J. Plant Physiol. 143, 534–537.

    CAS  Google Scholar 

  24. Cossins, A. R. (1994), Temperature Adaptation of Biological Membranes, Portland Press, London.

    Google Scholar 

  25. Los, D. A., Ray, M. K., and Murata, N. (1997), Mol. Microbiol. 25, 1167–1175.

    Article  PubMed  CAS  Google Scholar 

  26. Sakamoto, T., Shen, G., Higashi, S., Murata, N., and Bryant, D. A. (1998), Arch. Microbiol. 169, 20–28.

    Article  PubMed  CAS  Google Scholar 

  27. Niima, Y. and Taguchi, S. (1966), Nippon Suisan Gakkaisi 32, 1037–1042.

    Google Scholar 

  28. Ando, H. and Kaneda, N. (1968), Eiyou to Shokuryou 21, 245–249.

    CAS  Google Scholar 

  29. Molina, G. E., Sanchez, P. J. A., Garcia, C. F., Garcia, S. J. L., and Fernandez, S. J. M. (1995), J. Appl. Phycol. 7, 129–134.

    Article  Google Scholar 

  30. Reis, A., Gouveia, L., Veloso, V., Fernandes, H. L., Empis, J. A., and Novais, J. M. (1996), Biores. Technol. 55, 83–88.

    Article  CAS  Google Scholar 

  31. Otero, A., Garcia, D., Morales, E. D., Aran, J., and Fabregas, J. (1997), Biotechnol. Appl. Biochem. 26, 171–177.

    CAS  Google Scholar 

  32. Kitano, M., Matsukawa, R., and Karube, I. (1998), J. Appl. Phycol. 10, 101–105.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Center for Advanced Science and Technology, The University of Tokyo, 153-8904, Tokyo, Japan

    Ritsuko Matsukawa & Isao Karube

  2. National Industrial Research Institute, Tohoku, 983-0036, Sendai-shi, Japan

    Kiyotaka Hatakeda & Shota Ito

  3. Department of Environmental Engineering, Saitama Institute of Technology, 369-0293, Saitama, Japan

    Yukiyo Numata, Hidetoshi Nakamachi, Yasushi Hasebe & Shunichi Uchiyama

  4. Laboratory of Applied Phycology, Tokyo University of Fisheries, 108-8477, Tokyo, Japan

    Masahiro Notoya

  5. Department of Life Sciences, Bar-Ilan University, 52900, Ramat-Gan, Israel

    Zvy Dubinsky

Authors
  1. Ritsuko Matsukawa
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  2. Kiyotaka Hatakeda
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  3. Shota Ito
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  4. Yukiyo Numata
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  5. Hidetoshi Nakamachi
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  6. Yasushi Hasebe
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  7. Shunichi Uchiyama
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  8. Masahiro Notoya
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  9. Zvy Dubinsky
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  10. Isao Karube
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Correspondence to Isao Karube.

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Matsukawa, R., Hatakeda, K., Ito, S. et al. Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation. Appl Biochem Biotechnol 80, 141–150 (1999). https://doi.org/10.1385/ABAB:80:2:141

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  • DOI: https://doi.org/10.1385/ABAB:80:2:141

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