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Optimization of L-Lactic acid production from glucose by Rhizopus oryzae ATCC 52311


The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 523 11. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration in a bubble column was conducted under optimal conditions. Results showed a maximum lactic-acid production rate of 2.58 g/L/h was obtained with an initial glucose concentration of 94 g/L. Finallactic-acid concentration of 83 g/L was achieved after 32 h of fermentation with a weight of 0.88 glactic acid/g glucose consumed.

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  1. 1.

    Tsai, S. P., Coleman, R. D., Moon, S. H., Schneider, K. A., and Millard, C. S. (1993), Appl. Biochem. Biotechnol. 39/40, 323–335.

    Google Scholar 

  2. 2.

    Soccol, C. R., Stonoga, V. J., and Raimbault, M. (1994), World J. Microbiol. Biotechnol. 10, 433–435.

    Article  CAS  Google Scholar 

  3. 3.

    Rosenberg, M. and Kristofikova, L. (1995), Acta Biotechnol. 15, 367–374.

    Article  CAS  Google Scholar 

  4. 4.

    Yu, R. C. and Hang, Y. D. (1989), Biotechnol. Lett. 11, 597–600.

    Article  CAS  Google Scholar 

  5. 5.

    Yang, C. W., Lu, Z., and Tsao, G. T. (1995), Appl. Biochem. Biotechnol. 51, 57–71.

    Google Scholar 

  6. 6.

    Lin, J., Chen, B., Wu, J., and Cen, P. (1997), Chinese J. Chem. Eng. 5, 49–55.

    Google Scholar 

  7. 7.

    Du, J., Cao, N., Gong, C. S., and Tsao, G. T. (1998), Appl. Biochem. Biotechnol. 70/72, 323–329.

    Google Scholar 

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Correspondence to Ningjun Cao.

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Zhou, Y., Domínguez, J.M., Cao, N. et al. Optimization of L-Lactic acid production from glucose by Rhizopus oryzae ATCC 52311. Appl Biochem Biotechnol 78, 401–407 (1999).

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Index Entries

  • Bubble column
  • lactic acid
  • Rhizopus oryzae