Applied Biochemistry and Biotechnology

, Volume 135, Issue 2, pp 133–144 | Cite as

Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein

  • Mi Young LeeEmail author
  • Yoon Kyoung Kim
  • Kun Kul Ryoo
  • Yoon Bae Lee
  • Eun Ju Park
Original Research Articles


The generation of reactive oxygen species is thought to cause extensive oxidative damage to various biomolecules such as DNA, RNA, and protein. In this study, the preventive, suppressive, and protective effects of in vitro supplementation with electrolyzed-reduced water on H2O2-induced DNA damage in human lymphocytes were examined using a comet assay. Pretreatment, cotreatment, and posttreatment with electrolyzed-reduced water enhanced human lymphocyte resistance to the DNA strand breaks induced by H2O2 in vitro. Moreover, electrolyzed-reduced water was much more effective than diethylpyrocarbonate-treated water in preventing total RNA degradation at 4 and 25°C. In addition, electrolyzed-reduced water completely prevented the oxidative cleavage of horseradish peroxidase, as determined using sodium dodecyl sulfate-polyacrylamide gels. Enhancement of the antioxidant activity of ascorbic acid dissolved in electrolyzed-reduced water was about threefold that of ascorbic acid dissolved in nonelectrolyzed deionized water, as measured by a xanthine-xanthine oxidase superoxide scavenging assay system, suggesting an inhibitory effect of electrolyzed-reduced water on the oxidation of ascorbic acid.

Index Entries

Electrolyzed-reduced water oxidative damage DNA RNA protein 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Mi Young Lee
    • 1
    Email author
  • Yoon Kyoung Kim
    • 1
  • Kun Kul Ryoo
    • 2
  • Yoon Bae Lee
    • 2
  • Eun Ju Park
    • 3
  1. 1.Department of Genetic EngineeringSoonchunhyang UniversityChungnamKorea
  2. 2.Division of Material and Chemical EngineeringSoonchunhyang UniversityChungnamKorea
  3. 3.Department of Food and Nutritional ScienceKyungnam UniversityMasanKorea

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