Abstract
The inflence of extremely low-frequency (ELF) electromagnetic fields on Escherichia coli cultures in submerse fermentation was studied. The fermentation processes were carried out recycling the culture medium externally through a stainless steel tube inserted in a magnetic field generator (solenoid). The exposure time and electromagnetic induction were varied in a range of 1 to 12 h and 0.010 to 0.10 T, respectively, according to a Box-Wilson Central Composite Designs of face centered with five central points. Growth of E. coli could be altered (stimulated or inhibited) under magnetic fieldinduced effects. E. coli culturesexposed at 0.1 T during 6.5 h exhibited changes in its viability compared to unexposed cells, which was 100 times higher than the control. The magnetic field generator associated with the cellular suspension recycle is a new way of magnetic treatment in fermentation processes and could be appropriate to industrial scale up.
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References
Lei, C. and Berg, H. (1998), Biolectroch. Bioener. 45, 261–265.
Nakasono, S. and Saiki, H. (2000), Radiat. Res. 154, 208–216.
Kohane, M. J. and Tiller, W. A. (2000), J. Sci. Explor. 14(2), 217–231.
Blackman, C. F., Benane, S. G., Elliott, D. J., and Pollock M. M (1988), Bioelectro-magnetics 9, 215–227.
Bauréus, C.L. M. K., Sommarin, M., Persson, B. R. R., Salford L. G., and Eberhardt, J. L. (2003), Bioelectromagnetics 24, 395–402.
Costato, M., Majni, G., Venturelli, T., and Bolognani, L. (1992), 9 Lecture. International Congress of EBEA, Brussels
Goodman, E. M., Greenebaum, B., and Marron, M. T. (1994), Bioelectromagnetics 15, 77–83.
Kropinski, A. M., Morris, C., and Szewczuk, M. R. (1994), Bioelectromagnetics 15, 283–291.
Chacón, D. A., Haber, V. P., Fong, A. R., Mas, S. D., Serguera, M. N., and Justo, O. R. (1996), Tecnol. Quim. 15, 52–60.
Ivanova, V., Hristov, J., and Dobreva, E. (1996), Appl. Biochem. Biotech. 59, 187–198.
Raja, R., Sonolikar, R. L., and Pentu, S. S. (1997), Chem Eng. Sci. 52, 4155–4160.
Nascimento, L. F. C., Botura Jr., G. and Mota, R.P. (2003), Rev. Inst. Med. Trop. 45, 65–67.
Melek, S. C., Gonzalez, A. M., Camue, H. C., Diaz, C. M. C., Pardo, A. M. T., Haber, V. P. and Fong, A. R. (1996), Tecnol. Quim. 16, 26–31.
Melek, S. C., Gonzalez, A. M., Camue, H. C., Diaz, C. M. C., Pardo, A. M. T., Haber, V. P., and Fong, A. R. (1996), Tecnol. Quim. 16, 32–39.
Alvarez, D. C., Perez, V. H., Justo, O. R., and Alegre, R. M. (2006), Process Biochem. in press.
NIST/SEMATECH e-Handbook of Statistical Methods, http://www.itl.nist.gov/ div898/ handbook/, September 11, 2005.
Potenza, L., Ubaldi, L, De Sanctis, R., De Bellis, R., Cucchiarini, L., and Dacha, M. (2004), Mutat. Res. 561, 53–62.
Box, G. E. P. and Draper, N. R. (1987), Empirical model-building and response surfaces, John Willey & Sons, NY.
Chizhov, S. V., Sinyak, Y. Y., Shikina, M. I., Ukhanova, S. I., and Krasnoshchikov, V. V. (1975), Space Biol. Acrosp. Med. 9, 42–48.
Li, M., Qu, J. H., and Peng, Y. Z. (2004), J. Environ. Sci. 16, 348–352.
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Rodriguez Justo, O., Haber Pérez, V., Chacon Alvarez, D. et al. Growth of Escherichia coli under extremely low-frequency electromagnetic fields. Appl Biochem Biotechnol 134, 155–163 (2006). https://doi.org/10.1385/ABAB:134:2:155
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DOI: https://doi.org/10.1385/ABAB:134:2:155