Applied Biochemistry and Biotechnology

, Volume 118, Issue 1–3, pp 243–251 | Cite as

Siderophore production by a marine Pseudomonas aeruginosa and its antagonistic action against phytopathogenic fungi

  • A. V. Manwar
  • S. R. Khandelwal
  • B. L. Chaudhari
  • J. M. Meyer
  • S. B. ChincholkarEmail author


A marine isolate of fluorescent Pseudomonas sp. having the ability to produce the pyoverdine type of siderophores under low iron stress (up to 10 µM iron in the succinate medium) was identified as Pseudomonas aeruginosa by using BIOLOG Breathprint and siderotyping. Pyoverdine production was optimum at 0.2% (w/v) succinate, pH 6.0, in an iron-deficient medium. Studies carried out in vitro revealed that purified siderophores and Pseudomonas culture have good antifungal activity against the plant deleterious fungi, namely, Aspergillus niger, Aspergillus flavus, Aspergillus oryzae, Fusarium oxysporum, and Sclerotium rolfsii. Siderophore-based maximum inhibition was observed against A. niger. These in vitro antagonistic actions of marine Pseudomonas against phytopathogens suggest the potential of the organism to serve as a biocontrol agent.

Index Entries

Marine fluorescent pseudomonas siderophores phytopathogens biocontrol agent pyoverdine 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Jaytilake, G. S., Thonton, M. P., Leonard, A. C., Grimwade, J. E., and Baker, B. J. (1996), J. Nat. Prod. 59(3), 293–296.CrossRefGoogle Scholar
  2. 2.
    Burgess, J. G., Jordan, E. M., Bregu, M., Mearns-Spragg, A., and Boyd, K. G. (1999), J. Biotechnol. 70(1–3), 27–32.PubMedCrossRefGoogle Scholar
  3. 3.
    Reid, I. T., Live, D. H., Faulkner, D. J., and Butler, A. (1993), Nature 366, 455–458.PubMedCrossRefADSGoogle Scholar
  4. 4.
    Guerinot, M. L. (1994), Annu. Rev. Microbiol. 48, 743–772.PubMedCrossRefGoogle Scholar
  5. 5.
    Bayer, B. R. (1986), in Siderophores and Biological Systems: An Overview, Iron Siderophore and Plant Diseases (NATO ASI Series), Series A, Life Sciences, vol. 117, Swineburne, T. R., ed., Plenum, New York, pp. 1–6.Google Scholar
  6. 6.
    Kloepper, J. W., Schroth, M. N., and Miller, T. D. (1980), Phytopathology 70, 1078–1087.Google Scholar
  7. 7.
    Cook, R. J. (1985), Phytopathology 75, 25–29.Google Scholar
  8. 8.
    Dileepkumar, B. S. and Bezbaruah, B. (1997), Indian J. Microbiol. 35, 289–292.Google Scholar
  9. 9.
    Chincholkar, S. B., Chaudhari, B. L., Talegaonkar, S. K., and Kothari, R. M. (2000), in Biocontrol Potential and Its Exploitation in Sustainable Agriculture, vol. 1, Upadhyay, R. K., Mukerji, K. G., and Chamola, P. C., eds., Kluwer Academic/Plenum Publishers, New York, pp. 49–70.Google Scholar
  10. 10.
    O’Sullivan, D. J. and O’Gara, F. (1992), Microbiol. Rev. 56, 662–676.PubMedGoogle Scholar
  11. 11.
    Budzikiewicz, H. (1993), FEMS Microbiol. Rev. 204, 209–228.Google Scholar
  12. 12.
    Johri, B. N., Rao, C. N. S., and Goel, R. (1997), in Biotechnological Approaches in Soil Microorganisms for Sustainable Crop Production, Dadarwal, K. R., ed., Scientific Publishers, Jodhpur, India, pp. 193–223.Google Scholar
  13. 13.
    Palleroni, N. J. (1984), in Bergey’s Manual of Systematic Bacteriology, vol.I, Krieg, N. R., ed., Williams & Wilkins, Baltimore, pp. 141–199.Google Scholar
  14. 14.
    Meyer, J. M., Stintzi, A., De Vos, D., Cornellis, P., Tappe, R., Taraz, K., and Budzikiewicz, H. (1997), Microbiology 143, 35–43.PubMedCrossRefGoogle Scholar
  15. 15.
    Meyer, J. M. and Abdallah, M. A. (1978), J. Gen. Microbiol. 107, 319–328.Google Scholar
  16. 16.
    Schwyn, B. and Neilands, J. B. (1987), Anal. Biochem. 160, 47–56.PubMedCrossRefGoogle Scholar
  17. 17.
    Payne, S. (1994), in Methods in Enzymology, vol. 235, Clark, V. L. and Bavoil, P. M., eds., Academic, New York, pp. 329–344.Google Scholar
  18. 18.
    Jalal, M. A. F. and Van der Helm, D. (1991), in Handbook of Microbial Iron Chelates, Winkelmann, G., ed., CRC Press, Boca Raton, FL, pp. 235, 236.Google Scholar
  19. 19.
    Csaky, T. (1948), Acta Chem. Scandinavica 2, 450–454.CrossRefGoogle Scholar
  20. 20.
    Arnow, L. E. (1937), J. Biol. Chem. 118, 531–537.Google Scholar
  21. 21.
    Stintzi, A. and Meyer, J. M. (1995), in Microbes for Better Living, Sankaran, R. and Manja, K. S., eds., MICON International, 94 DFRL, Mysore, India, pp. 169–176.Google Scholar
  22. 22.
    Manwar, A. V. (2001), PhD Thesis, North Maharashtra University, Jalgaon, India.Google Scholar
  23. 23.
    Jensen, P. R. and Fenical, W. (1994), Annu. Rev. Microbiol. 48, 559–584.PubMedCrossRefGoogle Scholar
  24. 24.
    Presmark, M., Frejd, T., and Mattiasson, B. (1990), Biochemistry 29, 7348–7356.CrossRefGoogle Scholar
  25. 25.
    Love, S. H. and Hulcher, F. H. (1964), J. Bacteriol. 87, 39–45.PubMedCrossRefGoogle Scholar
  26. 26.
    Chaudhari, B. L. (1999), PhD thesis, North Maharashtra University, Jalgaon, India.Google Scholar
  27. 27.
    Sindhu, S. S., Suneja, S., and Dadarwal, K. R. (1997), in Biotechnological Approaches in Soil Microorganisms for Sustainable Crop Production, Dadarwal, K. R., ed., Scientific Publishers, Jodhpur, India, pp. 149–193.Google Scholar
  28. 28.
    Manwar, A. V., Vaigankar, P. D., Bhoge, L. S., and Chincholkar, S. B. (2000), Indian J. Microbiol. 40(2), 109–112.Google Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • A. V. Manwar
    • 1
  • S. R. Khandelwal
    • 1
  • B. L. Chaudhari
    • 1
  • J. M. Meyer
    • 2
  • S. B. Chincholkar
    • 1
    Email author
  1. 1.Department of Microbiology, School of Life SciencesNorth Maharashtra UniversityJalgaonIndia
  2. 2.Laboratoire de Microbiologie et de GenetiqueLouis Pasteur Université, No. 1481StrasbourgFrance

Personalised recommendations