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Applied Biochemistry and Biotechnology

, Volume 118, Issue 1–3, pp 243–251 | Cite as

Siderophore production by a marine Pseudomonas aeruginosa and its antagonistic action against phytopathogenic fungi

  • A. V. Manwar
  • S. R. Khandelwal
  • B. L. Chaudhari
  • J. M. Meyer
  • S. B. ChincholkarEmail author
Article

Abstract

A marine isolate of fluorescent Pseudomonas sp. having the ability to produce the pyoverdine type of siderophores under low iron stress (up to 10 µM iron in the succinate medium) was identified as Pseudomonas aeruginosa by using BIOLOG Breathprint and siderotyping. Pyoverdine production was optimum at 0.2% (w/v) succinate, pH 6.0, in an iron-deficient medium. Studies carried out in vitro revealed that purified siderophores and Pseudomonas culture have good antifungal activity against the plant deleterious fungi, namely, Aspergillus niger, Aspergillus flavus, Aspergillus oryzae, Fusarium oxysporum, and Sclerotium rolfsii. Siderophore-based maximum inhibition was observed against A. niger. These in vitro antagonistic actions of marine Pseudomonas against phytopathogens suggest the potential of the organism to serve as a biocontrol agent.

Index Entries

Marine fluorescent pseudomonas siderophores phytopathogens biocontrol agent pyoverdine 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • A. V. Manwar
    • 1
  • S. R. Khandelwal
    • 1
  • B. L. Chaudhari
    • 1
  • J. M. Meyer
    • 2
  • S. B. Chincholkar
    • 1
    Email author
  1. 1.Department of Microbiology, School of Life SciencesNorth Maharashtra UniversityJalgaonIndia
  2. 2.Laboratoire de Microbiologie et de GenetiqueLouis Pasteur Université, No. 1481StrasbourgFrance

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