Applied Biochemistry and Biotechnology

, Volume 116, Issue 1–3, pp 1193–1203 | Cite as

Effects of inoculum conditions on growth of hairy roots of Panax ginseng C. A. Meyer

  • Gwi-Taek Jeong
  • Don-Hee ParkEmail author
  • Hwa-Won Ryu
  • Baik Hwang
  • Je-Chang Woo
Session 6B— Plant Biotechnology and Feedstock Genomics


Plants have a potential to produce a large number of important metabolites such as pharmaceuticals, food additives, pigments, flavors, fragrances, and fine chemicals. Large-scale plant cell and tissue cultures for producing useful products has been considered an attractive alternative to whole plant extraction for obtaining valuable chemicals. In plant cell and tissue cultures, cell growth and metabolite production are influenced by nutritional and environmental conditions as well as physical properties of the culture system. To obtain a high growth rate of plant cell and tissue cultures, the culture tem. To obtain a high growth rate of plant cell and tissue cultures, the culture conditions should be maintained at an optimum level. We studied the relationship between inoculum conditions and the growth of Panax ginseng hairy root culture, and found that the growth rate varied with the inoculum conditions such as the number of root tips, the length of root tips, the part of root tips, and the inoculum size and age of hairy roots.

Index Entries

Panax ginseng transformed hairy root inoculum condition inoculum age root culture 


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  1. 1.
    Banthorpe, D. V. (1994), Nat. Prod. Rep. 11, 303–328.CrossRefGoogle Scholar
  2. 2.
    Chattopadhyay, S., Farkya, S., Srivastava, A. K., and Bisaria, V. S. (2002), Biotechnol. Bioprocess Eng. 7(3), 138–149.Google Scholar
  3. 3.
    Prakash, G., Bhojwani, S. S., and Srivastava, A. K. (2002), Biotechnol. Bioprocess Eng. 7(4), 185–193.Google Scholar
  4. 4.
    Giri, A. and Narasu, M. L. (2000), Biotechnol. Adv. 18, 1–22.CrossRefGoogle Scholar
  5. 5.
    Nilsson, O. and Olsson, O. (1997), Physiologia Plantarum 100, 463–473.CrossRefGoogle Scholar
  6. 6.
    Nobuyuki, V. and Takesh, K. (1994), in Advances in Plant Biotechnology, Ryu, D. D. Y. and Furusaki, S., eds., Elsevier, Amsterdam, pp. 307–338.Google Scholar
  7. 7.
    Jeong, G. T., Park, D. H., Hwang, B., and Woo, J. C. (2003), Appl. Biochem. Biotechnol. 105–108, 493–503.CrossRefGoogle Scholar
  8. 8.
    Bais, H. P., Suresh, B., Raghaoarao, K. S. M. S., and Ravishankar, G. A. (2002), In Vitro Cell. Dev. Biol.-Plant. 38, 573–580.CrossRefGoogle Scholar
  9. 9.
    Liu, C. Z., Guo, C., Wang, Y. C., and Ouyang, F. (2003), Process Biochem. 39, (1), 45–49.CrossRefGoogle Scholar
  10. 10.
    Takahashi, Y., Hitaka, Y., Kino-Oka, M., Taya, M., and Tone, S. (2001), Biochem. Eng. J. 8, 121–127.CrossRefGoogle Scholar
  11. 11.
    Kanokwaree, K. and Doran, P. M. (1997), J. Ferment. Bioeng. 84(4), 378–381.CrossRefGoogle Scholar
  12. 12.
    Falk, L. R. and Doran, P. M. (1996), Biotechnol. Lett. 18(9), 1099–1104.CrossRefGoogle Scholar
  13. 13.
    Ramakrishnan, D., Salim, J., and Curtis, W. R. (1994), Biotechnol. Tech 8(9), 639–644.CrossRefGoogle Scholar
  14. 14.
    Carvalho, E. B. and Curtis, W. R. (1999), Biotechnol. Bioprocess Eng. 4, 287–293.CrossRefGoogle Scholar
  15. 15.
    Bhadra, R. and Shanks, J. V. (1995), Biotechnol. Tech. 9(9) 681–686.CrossRefGoogle Scholar
  16. 16.
    Jung, N. P. and Jin, S. H. (1996), Korean J. Ginseng Sci. 20(4), 431–471.Google Scholar
  17. 17.
    Wu, J. and Zhong, J. J. (1999), J. Biotechnol. 68, 88–99.CrossRefGoogle Scholar
  18. 18.
    Jeong, G. T., Park, D. H., Ryu, H. W., Lee, W. T., Park, K., Kang, C. H., Hwang, B., and Woo, J. C. (2002), Appl. Biochem. Biotechnol. 98–100, 1129–1139.CrossRefGoogle Scholar
  19. 19.
    Kino-Oka, M., Hitaka, Y., Niomiya, K., Taya, M., and Tone, S. (1999), J. Biosci. Bioeng. 88(6), 690–692.CrossRefGoogle Scholar
  20. 20.
    Woo, S. H., Park, J. M., and Yang, J. W. (1997), Plant Cell Tissue Organ Cult. 48, 131–134.CrossRefGoogle Scholar
  21. 21.
    Zhang, C. H., Wu, J. H., and He, G. Y. (2002), Appl. Microbiol. Biotechnol. 60, 396–402.CrossRefGoogle Scholar
  22. 22.
    Pavlov, A., Georgiev, V., and Kovatcheva, P. (2003), Biotechnol. Lett. 25, 307–309.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Gwi-Taek Jeong
    • 1
  • Don-Hee Park
    • 1
    • 2
    Email author
  • Hwa-Won Ryu
    • 1
  • Baik Hwang
    • 3
  • Je-Chang Woo
    • 4
  1. 1.Faculty of Applied Chemical EngineeringChonnam National UniversityKwangjuKorea
  2. 2.Institute of Bioindustrial TechnologyChonnam National UniversityKwangjuKorea
  3. 3.Department of Biological SciencesChonnam National UniversityKwangjuKorea
  4. 4.Department of BiologyMokpo National UniversityChonnamKorea

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