Skip to main content
SpringerLink
Log in

Effects of sugar inhibition on cellulases and β-glucosidase during enzymatic hydrolysis of softwood substrates

  • Session 6A Biomass Pretreatment and Hydrolysis
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A quantitative approach was taken to determine the inhibition effects of glucose and other sugar monomers during cellulase and β-Glucosidase hydrolysis of two types of cellulosic material: Avicel and acetic acid-pretreated softwood. The increased glucose content in the hydrolysate resulted in a dramatic increase in the degrees of inhibition on both β-Glucosidase and cellulase activities. Supplementation of mannose, xylose, and galactose during cellobiose hydrolysis did not show any inhibitory effects on β-Glucosidase activity. However, these sugars were shown to have significant inhibitory effects on cellulase activity during cellulose hydrolysis. Our study suggests that high-substrate consistency hydrolysis with supplementation of hemicellulose is likely to be a practical solution to minimizing end-product inhibition effects while producing hydrolysate with high glucose concentration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Galbe, M. and Zacchi, G. (2002), Appl. Microbiol. Biotechnol. 59, 618–628.

    Article  PubMed  CAS  Google Scholar 

  2. Sun, Y. and Cheng, J. Y. (2002), Bioresour. Technol. 83, 1–11.

    Article  PubMed  CAS  Google Scholar 

  3. Kim, J., Seok, L. Y. Y., and Torget, R. W. (2001), Appl. Biochem. Biotechnol. 91–93, 331–340.

    Article  PubMed  Google Scholar 

  4. Duff, S. J. B. and Murray, W. D. (1996), Bioresour. Technol. 55, 1–33.

    Article  CAS  Google Scholar 

  5. Hsu, T. (1996), Handbook of Bioethanol: Production and Utilization, Wyman, C. E., ed., Taylor & Francis, Washington, DC, pp. 179–212.

    Google Scholar 

  6. Gusakov, A. V. and Sinitsyn, A. P. (1992), Biotechnol. Bioeng. 40, 663–671.

    Article  CAS  Google Scholar 

  7. Dekker, R. F. H. (1986), Biotechnol. Bioeng. 28, 1438–1442.

    Article  CAS  Google Scholar 

  8. Holtzapple, M., Cognata, M., Shu, Y., and Hendrickson, C. (1990), Biotechnol. Bioeng. 36, 275–287.

    Article  CAS  Google Scholar 

  9. Ramos, L. P., Breuil, C., and Saddler, J. N. (1993), Enzyme Microbial. Technol. 15, 19–25.

    Article  CAS  Google Scholar 

  10. Gregg, D. J. and Saddler, J. N. (1996), Biotechnol. Bioeng. 51, 375–383.

    Article  CAS  Google Scholar 

  11. Ohmine, K., Ooshima, H., and Harano, Y. (1983), Biotechnol. Bioeng. 25, 2041–2053.

    Article  CAS  Google Scholar 

  12. Holtzapple, M. T., Caram, H. S., and Humphrey, A. E. (1984), Biotechnol. Bioeng. 26, 753–757.

    Article  CAS  Google Scholar 

  13. Scheiding, W., Thoma, M., Ross, A., and Schuegerl, K. (1984), Appl. Microbiol. Biotechnol. 20, 176–182.

    Article  CAS  Google Scholar 

  14. Tengborg, C., Galbe, M., and Zacchi, C. (2001), Enzyme Microbial. Technol. 28, 835–844.

    Article  CAS  Google Scholar 

  15. Wood, T. M. and Bhat, K. M. (1988), in Methods in Enzymology, vol. 160, Wood, W. and Kellogg, S., eds., Academic, New York, NY, pp. 87–112.

    Google Scholar 

  16. Boussaid, A. L., Esteghlalian, A. R., Gregg, D. J., Lee, K. H., and Saddler, J. N. (2000), Appl. Biochem. Biotechnol. 84–86, 693–705.

    Article  PubMed  Google Scholar 

  17. Gong, C.-S., Ladisch, M. R., Tsao, G. T. (1977), Biotechnol. Bioeng. 19, 959–981.

    Article  PubMed  CAS  Google Scholar 

  18. Montero, M. and Romeu, A. (1992), Appl. Microbiol. Biotechnol. 38, 350–353.

    Article  CAS  Google Scholar 

  19. Yeoh, H. H., Tan, T. K., and Koh, S. K. (1986), Appl. Microbiol. Biotechnol. 25, 25–28.

    Article  CAS  Google Scholar 

  20. Asenjo, J. A. (1983), Biotechnol. Bioeng. 25, 3185–3190.

    Article  CAS  Google Scholar 

  21. Rao, M., Seeta, R., and Deshpande, V. (1989), Biotechnol. Appl. Biochem. 11, 477–482.

    CAS  Google Scholar 

  22. Beltrame, P. L., Carniti, P., Focher, B., Marzetti, A., and Sarto, V. (1984), Biotechnol. Bioeng. 26, 1233–1238.

    Article  CAS  Google Scholar 

  23. Mosolova, T. P., Kalyuzhnyi, S. V., Varfolomeyev, S. D., and Velikodvorskaya, G. A. (1993), Appl. Biochem. Biotechnol. 42, 9–18.

    PubMed  CAS  Google Scholar 

  24. Hadj-Taieb, N., Chaabouni-Ellouz, S., Kammoun, A., and Ellouz, R. (1992), Appl. Microbiol. Biotechnol. 37, 197–201.

    Article  CAS  Google Scholar 

  25. Murray, W. D. (1987), Biotechnol. Bioeng. 29, 1151–1154.

    Article  CAS  Google Scholar 

  26. Breuil, C., Chan, M., and Saddler, J. N. (1990), Appl. Microbiol. Biotechnol. 34, 31–35

    Article  CAS  Google Scholar 

  27. Dekker, F. F. H. and Wallis, A. F. A. (1983), Biotechnol. Bioeng. 25, 3027–3048.

    Article  CAS  Google Scholar 

  28. Zacchi, G. and Axelsson, A. (1989), Biotechnol. Bioeng. 34, 223–233.

    Article  CAS  Google Scholar 

  29. Larsson, M., Galbe, M., and Zacchi, G. (1997), Bioresourc. Technol. 60, 143–151.

    Article  CAS  Google Scholar 

  30. Saha, B. C., Freer, S. N., and Bothast, R. J. (1994), Appl. Environ. Microbiol. 60, 3774–3780.

    PubMed  CAS  Google Scholar 

  31. Yun, S. I., Jeong, C.-S., Chung, D.-K., and Choi, H.-S. (2001), Biosci. Biotechnol. Biochem. 65, 2028–2032.

    Article  PubMed  CAS  Google Scholar 

  32. Klyosov, A. A., Sinitsyn, A. P., and Rabinowitch, M. L. (1980), Enzyme Eng. 5, 153–165.

    CAS  Google Scholar 

  33. Sjostrom, E. (1981) Wood Chemistry: Fundamentals and Applications, Academic, San Diego, CA.

    Google Scholar 

  34. Fengel, D. and Wegener, G. (1984), Wood: Chemistry, Ultrastructure, Reactions, Walter de Gruyter, Berlin, Germany.

    Google Scholar 

Download references

Author information

Author notes

  1. Xiao Zhang

    Present address: Paprican, 570 St. Jean Blvd., H9R 3JR, Pointe Claire, Quebec, Canada

Authors and Affiliations

  1. Forest Products Biotechnology, University of British Columbia, 4033-2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada

    Zhizhuang Xiao, Xiao Zhang, David J. Gregg & John N. Saddler

Authors
  1. Zhizhuang Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David J. Gregg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John N. Saddler
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to John N. Saddler.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xiao, Z., Zhang, X., Gregg, D.J. et al. Effects of sugar inhibition on cellulases and β-glucosidase during enzymatic hydrolysis of softwood substrates. Appl Biochem Biotechnol 115, 1115–1126 (2004). https://doi.org/10.1385/ABAB:115:1-3:1115

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:115:1-3:1115

Index Entries

Search

Navigation