Abstract
An energy-efficient hollow-fiber membrane extraction process was successfully developed to separate and recover lactic acid produced in fermentation. Although many fermentation processes have been developed for lactic acid production, and economical method for lactic acid recovery from the fermentation broth is still needed. Continuous extraction of lactic acid from a simulated aqueous stream was achieved by using Alamine 336 in 2-octanol contained in a hollow-fiber membrane extractor. In this process, the extractant was simultaneously regenerated by stripping with NaOH in a second membrane extractor, and the final product is a concentrated lactate salt solution. The extraction rate increased linearly with an increase in the Alamine 336 content in the solvent (from 5 to 40%). Increasing the concentration of the undissociated lactic acid in the feed solution by either increasing the lactate concentration (from 5 to 40 g/L) or decreasing the solution pH (from 5.0 to 4.0) also increased the extraction rate. Based on these observations, a reactive extraction model with a first-order reaction mechanism for both lactic acid and amine concentrations was proposed. The extraction rate also increased with an increase in the feed flow rate, but not the flow rates of solvent and the stripping solution, suggesting that the process was not limited by diffusion in the liquid films or membrane pores. A mathematical model considering both diffusion and chemical reaction in the extractor and back extractor was developed to simulate the process. The model fits the experimental data well and can be used in scale up design of the process.
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References
Clary, J. J., Feron, V. J., and van Velthuijsen, J. A. (1998), Regul. Toxicol. Pharm. 27, 88–97.
Nikles, S. M., Piao, M., Lane, A. M., and Nikles, D. E (2001), Green Chem. 3, 109–113.
Datta, R., Tsai, S. P., Bonsignore, P., Moon, S. H., and Frank, J. R. (1995), FEMS Microbiol. Rev. 16, 221–231.
Drumright, R. E., Gruber, P. R., and Henton, D. E. (2000), Adv. Mater, 12, 1841–1846.
Wasewar, K. L., Heesink, A. B. M., Versteeg, G. F., and Pangarkar, V. G. (2002), J. Chem. Technol. Biol. 77 1068–1075.
Holten, C. H. (1971), Lactic Acid, Verlag Chemie GmbH, Weinheim, Germany.
Tay, A. and Yang, S. T. (2002), Biotechnol. Bioeng. 80, 1–12.
Mulligan, C. N., Safi, B. F., and Grolea, U. D. (1991), Biotechnol. Bioeng. 38 1173–1181
Tsai, S. P. and Moon, S. H. (1998), Appl. Biochem. Biotechnol. 70, 417–428.
Jin, Z. W. and Yang, S. T. (1998), Biotechnol. Prog. 14, 457–465.
Wu, Z. T., and Yang, S. T. (2003), Biotechnol. Bioeng. 82, 93–102.
Tay, A. (2002), PhD thesis, Ohio State University, Columbus, OH.
Yang, C. W., Lu, Z. J., and Tsao, G. T. (1995), Appl. Biochem. Biotechnol. 51, 57–71.
Vonktaveesuk, P., Tonokawa, M., and ishizaki, A. (1994), J. Ferment. Bioeng. 77, 508–512.
Xuemei, L., Jianping, L., Móe, L., and Peilin, C. (1999), Bioprocess Eng. 20, 231–237.
Srivastava, A., Roychoudhury, P. K., and sahai, V. (1992), Biotechnol. Bioeng. 39, 607–613.
Wang, J. L., Wen, X. H., and Zhou, D. (2000), Bioresour. Technol. 75, 231–234.
Wang, J. L., Liu, P., and Zhou, D. (1994), Biotechnol. Tech. 8, 905–908.
Planas, J., Radstrom, P., Tjerneld, F., and HahnHagerdal, B. (1996), Appl. Microbiol. Biotechnol. 45, 737–743.
Kwon, Y. J., Kaul, R., and Mattiasson B. (1996), Biotechnol. Bioeng. 50, 280–290.
Kertes, A. S., and King, C. J. (1986), Biotechnol. Bioeng. 28, 269–282.
Lewis, V. P. and Yang, S. T. (1992), Biotechnol. Prog. 8, 104–110.
Lazarova, Z., Syska, B., and Schugerl K. (2002), J. Membr. Sci. 202, 151–164.
Reid, R. C., Prausnitz, J. M. and Sherwood, T. K. (1987), The Properties of Gases and Liquids, 4th Ed., McGraw-Hill, New York, NY.
San-Martin, M., Pazos, C., and Coca, J. (1992), J. Chem. Technol. Biotechnol. 54, 1–6.
Yang, S. T., White, S. A., and Hsu, S. T. (1991), Ind. Eng. Chem. Res. 30, 1335–1343.
Basu, R. and Sirkar, K. K. (1992), Solvent Extr. Ion Exch. 10, 119–142
Coelhoso, I. M., Silvestre, P., Viegas, R. M. C., Crespo, J. P. S. G., and Carrondo, M. J. T. (1997), J. Membr. Sci. 134 19–32.
Juang, R. S., Chen, J. D., and Huang, H. C. (2000), J. Membr. Sci. 165, 59–73.
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Huang, H., Yang, ST. & Ramey, D.E. A hollow-fiber membrane extraction process for recovery and separation of lactic acid from aqueous solution. Appl Biochem Biotechnol 114, 671–688 (2004). https://doi.org/10.1385/ABAB:114:1-3:671
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DOI: https://doi.org/10.1385/ABAB:114:1-3:671