Abstract
Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted.
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Rudolf, A., Galbe, M. & Lidén, G. Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale. Appl Biochem Biotechnol 114, 601–617 (2004). https://doi.org/10.1385/ABAB:114:1-3:601
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DOI: https://doi.org/10.1385/ABAB:114:1-3:601