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Enzymatic synthesis of monolaurin

  • Session 2—Introduction to Microbial Catalysis and Engineering
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Abstract

The aim of this study was to produce monolaurin utilizing a commercial immobilized lipase (Lipozyme IM-20; Novo Nordisk, Bagsvaerd, Denmark) through the direct esterification of lauric acid and glycerol in a solvent-free system. The influence of fatty acid/glycerol molar ratio, temperature, and Lipozyme (IM-20) concentration on the molar fraction of monolaurin were determined using an experimental design. The best conditions employed were 55°C, lauric acid/glycerol molar ratio of 1.0, and 3.0% (w/w) enzyme concentration. The final product, obtained after 6 h of reaction, was 45.5% monolaurin, 26.8% dilaurin, 3.1% trilaurin, and 24.6% lauric acid. The reusability of the enzyme was also studied.

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Authors and Affiliations

  1. Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Lab I 221, Cidade Univrsitária, CEP: 21949-900, RJ, RJ, Brazil

    Carla C. B. Pereira & Mônica A. P. da Silva

  2. Instituto de Química, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier 524, PHLC, sl 310/lab.427, CEP: 20550-013, RJ, RJ, Brazil

    Marta A. P. Langone

Authors
  1. Carla C. B. Pereira
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  2. Mônica A. P. da Silva
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  3. Marta A. P. Langone
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Correspondence to Marta A. P. Langone.

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Pereira, C.C.B., da Silva, M.A.P. & Langone, M.A.P. Enzymatic synthesis of monolaurin. Appl Biochem Biotechnol 114, 433–445 (2004). https://doi.org/10.1385/ABAB:114:1-3:433

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  • DOI: https://doi.org/10.1385/ABAB:114:1-3:433

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