Abstract
We have attempted to develop an active selection system for strains that have a higher potential for Avicel degradation using haploidized conidia from colchicine-treated Trichoderma reesei Rut C-30 as a model strain. Avicel, absorbent cotton, and wood powder were used as substrates for selection. It appeared that the strains that degrade Avicel actively could be effectively selected when the solid medium containing the selection substrate and the liquid medium containing Avicel were used.
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Hayward, T. K., Hamilton, J., Tholudur, A., and Mcmillan, J. D. (2000), Appl. Biochem. Biotechnol. 84/86, 859–874.
Heikinheimo, L., Buchert, J., Miettinen-Oinonen, A., and Suominen, P. (2000), Text. Res. J. 70, 969–973.
Ju, L-K. and Afolabi, O. A. (1999), Biotechnol. Prog. 15, 91–97.
Toyama, H. and Toyama, N. (2000), Appl. Biochem. Biotech. 84/86, 419–429.
Mandels, M. and Sternberg, D. (1976), J. Ferment. Technol. 54, 267–286.
Zelesco, P. A., Barbieri, I., and Graves, J. A. M. (1990), Mutat. Res. 242, 329–335.
Unbehaun, H., Dittler, B., Kuehne, G., and Wagenfuehr, A. (2000), Acta Biotechnol. 20, 305–312.
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Toyama, H., Yamagishi, N. & Toyama, N. Rapid selection system of strains with higher avicel degrading ability in a cellulolytic fungus, Trichoderma . Appl Biochem Biotechnol 98, 257–263 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:257
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DOI: https://doi.org/10.1385/ABAB:98-100:1-9:257