Abstract
In a 5-L fermentor (NBS-MF 105), Saccharomyces cerevisiae (0.7 g/L) was inoculated into a liquid medium (pH 4.0) containing 17 g/L of glucose, 2.55 g/L of yeast extract, 4.25 g/L of peptone, 2.04 g/L of Na2HPO4·12H2O, 4.34 g/L of (NH4)2SO4 and 0.064 g/L of MgSO4·7H2O and aerobically cultivated at 35°C for 22 h. Agitation and aeration were adjusted to attain initial k L a values of 15, 60, 135, and 230 h−1. The glucose 6-phosphate dehydrogenase (G6PDH) productivity (Pr G6PDH) obtained for kLa values of 15, 60, 135, and 230 h−1 was 10.6, 31.8, 30.3, and 23.3 U/([L·h]), respectively, whereas the cell productivity (Pr x) for the same k L a values were 0.24, 0.69, 0.69, and 0.49 g/[L·h], respectively. Thus, both events are coupled and depend on the dissolved oxygen in the medium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergmeyer, H. U. (1984), Methods of Enzymatic Analysis, 3rd ed., Verlag Chimie, Winheim, Germany.
Whitaker, J. R. (1991), Food Enzymology, Elsevier Applied Science, NY.
Godfrey, T. and West, S. (1996), Industrial Enzymology, 2nd ed. MacMillan Press, London.
White, J. (1954), Yeast Technology, Chapman and Hall, London.
Wise, W. S. (1951), J. Gen. Microbiol. 5, 167–177.
Joslyn, M. A. (1970), Methods in Food Analysis, 2nd ed., Academic Press, NY.
Le Duy, A.; Zajic, E. J. (1973), Biotechnol. Bioeng. 15, 805–810.
Borzani, W. (1975), in Engenharia Bioquímica, vol. 3, Borzani, W., Lima, U.A., and Aquarone, E., eds., Universidade de São Paulo, São Paulo, pp. 168–184.
Luedeking, R. and Piret, E. L. (1959), J. Biochem. Microbiol. Technol. Eng. 1, 393–401.
Garrett, R. H. and Grisham, C. M. (1995), Biochemistry, Saunders College Publishing, San Diego, CA.
Abrahão-Neto, J., Infanti, P., and Vitolo, M. (1996), Applied Biochemistry and Biotechnology 57/58, 407–412.
Barman, T. E. (1969), Enzyme Handbook vol.2, Spring-Verlag, NY.
Rose, A. H. and Harrison, J. S. (1989), The Yeasts, 2nd ed., Academic Press, NY.
Rehm, H. J., and Reed, G. (1993), Biotechnology, 2nd ed., VCH, Weinheim, Germany.
Vitolo, M., Vairo, M. L. R., and Borzani, W. (1985), Biotechnol. Bioeng. 27, 1229–1235.
Doin, P. A. (1975), in Engenharia Bioquímica, vol.3, Borzani, W., Lima, U. A., and Aquarone, E., eds., Universidade de São Paulo, São Paulo, pp. 112–134.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pereira Silva, D., Pessoa, A., Roberto, I.C. et al. Effect of k L a on the production of glucose 6-phosphate dehydrogenase from Saccharomyces cerevisiae grown by fermentation process. Appl Biochem Biotechnol 98, 205–213 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:205
Issue Date:
DOI: https://doi.org/10.1385/ABAB:98-100:1-9:205