Applied Biochemistry and Biotechnology

, Volume 135, Issue 1, pp 43–57

Mutagenesis and analysis of mold Aspergillus niger for extracellular glucose oxidase production using sugarcane molasses

  • O. V. Singh
Original Research Articles

DOI: 10.1385/ABAB:135:1:43

Cite this article as:
Singh, O.V. Appl Biochem Biotechnol (2006) 135: 43. doi:10.1385/ABAB:135:1:43


Aspergillus niger ORS-4.410, a mutant of A. niger ORS-4, was generated by repeated ultraviolet (UV) irradiation. Analysis of the UV treatment dose on wild-type (WT) A. niger ORS-4, conidial survival, and frequency of mutation showed that the maximum frequency of positive mutants (25.5%) was obtained with a 57% conidial survival rate after the second stage of UV irradiation. The level of glucose oxidase (GOX) production from mutant A. niger ORS-4.410 thus obtained was 149% higher than that for WT strain A. niger ORS-4 under liquid culture conditions using hexacyanoferrate (HCF)-treated sugarcane molasses (TM) as a cheaper carbohydrate source. When subcultured monthly for 24 mo, the mutant strain had consistent levels of GOX production (2.62±0.51 U/mL). Mutant A. niger ORS-4.410 was markedly different from the parent strain morphologically and was found to grow abundantly on sugarcane molasses. The mutant strain showed 3.43-fold increases in GOX levels (2.62±0.51 U/mL) using HCF-TM compared with the crude form of cane molasses (0.762±0.158 U/mL).

Index Entries

Glucose oxidase sugarcane molasses submerged fermentation Aspergillus niger mutation 

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • O. V. Singh
    • 1
  1. 1.Department of BiotechnologyIndian Institute of TechnologyRoorkeeIndia
  2. 2.Department of PediatricsThe Johns Hopkins School of MedicineBaltimore

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