Abstract
Foam fractionation cannot be used to recover cellulase from an aerated water solution effectively because cellulase by itself can produce only a small amount of foam. The addition of a surfactant can, however, increase the foamate volume and enhance the concentration of cellulase. We studied three detergents individually added to a 200 mg/L cellulase solution to promote foaming. These detergents were anionic, cationic, and nonionic surfactants, respectively. Although contributing to foam production, it was observed that nonionic surfactant (Pluronic F-68) barely concentrated cellulase, leaving the enrichment ratio unchanged, near 1. With anionic surfactant, sodium dedecyl sulfate, and cationic surfactant, cetyltrimethylammonium bromide (CTAB), the enrichment ratio became much larger, but cellulase denaturation occurred, reducing the activity of the enzyme. When CTAB was used to help foam cellulase, β-cyclodextrin was subsequently added to the foamate to help restore the enzyme activity.
Similar content being viewed by others
Reference
Johansson, G. and Reczey, K. (1998), J. Chromatogr B 711(1–2), 161–172.
London, M., Cohen, M., and Hudson, P. B. (1954), Biochim. Biophys. Acta 13, 111–120.
Schnepf, R. W. and Gaden, E. L. (1959), J. Biochem. Microbiol. Technol. Eng. 1(1), 1–8.
Suzuki, A., Yasuhara, K., Seki, H., and Maruyama, H. (2002), J. Colloid Interface Sci. 253(2), 402–408.
Clarkson, J. R., Cui, Z. F., and Darton, R. C. (1999), J. Colloid Interface Sci. 215(2), 323–332.
Clarkson, J. R., Cui, Z. F., and Darton, R. C. (1999), J. Colloid Interface Sci. 215(2), 333–338.
Chi, E. Y., Krishnan, S., Randolph, T. W., and Carpenter, J. F. (2003), Pharma. Res. 20(9), 1325–1336.
Randolph, T. W., and Jones, L. S. (2002), in Rational Design of Stable Protein Formulations, J. F. Carpenter and M. L. Manning, eds., Kluwer Academic/Plenum, New York, pp. 159–175.
Middelberg, A. R. (2002), Trends Biotechnol. 20(10), 437–443.
Clark, E. D. B. (2001), Curr. Opin. Biotechnol. 12(2), 202–207.
Voet, D., Voet, J. G., and Pratt, C. W. (1999), Fundamentals of Biochemistry, John Wiley & Sons, New York, pp. 154–157.
Rozema, D. and Gellman, S. H. (1995), J. Am. Chem. Soc. 117(8), 2373–2374.
Machida, S., Ogawa, S., Shi, X. H., Takaha, T., Fujii, K., and Hayashi, K., (2000), FEBS Lett. 486(2), 131–135.
Rozema, D. and Gellman, S. H. (1996), Biochemistry 35(49), 15,760–15,771.
Sundari, C.S., Raman, B., and Balasubramanian, D. (1999), FEBS Lett. 443(2), 215–219.
Kuboi, R., Mawatari, T., and Yoshimoto, M. (2000), J. Biosci. Bioeng. 90(1), 14–19.
Dong, X. Y., Shi, J. H., and Sun, Y. (2002), Biotechnol. Prog. 18(3), 663–665.
Daugherty, D. L., Rozema, D., Hanson, P. E., and Gellman, S. H. (1998), J. Biol. Chem. 273(51), 33,961–33,971.
Couthon, F., Clottes, E., and Vial, C. (1996), Biochem. Biophys. Res. Comm. 227(3), 854–860.
Nath, D. and Roa, M. (2001), Eur. J. Biochem. 268, 5471–5478.
Kim, C. S. and Lee, E. K. (2000), Process Biochem. 36(1–2), 111–117.
Mandels, M., Anderotti, R., and Roche, C. (1976), Biotechnol. Bioeng. Symp. 6, 21–33.
Almin, K. and Eriksson, K. (1968), Arch. Biochem. Biophys. 124(129).
Loha, V., Prokop, A., Du, L. P., and Tanner, R. D. (1999), Appl. Biochem. Biotechnol. 77–9, 701–712.
Brown, A.K., Kaul, A., and Varley, J. (1999), Biotechnol. Bioeng. 62(3), 278–290.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Burapatana, V., Prokop, A. & Tanner, R.D. Enhancing cellulase foam fractionation with addition of surfactant. Appl Biochem Biotechnol 122, 541–552 (2005). https://doi.org/10.1385/ABAB:122:1-3:0541
Issue Date:
DOI: https://doi.org/10.1385/ABAB:122:1-3:0541