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Effect of media on spore yield and thermal resistance of Bacillus stearothermophilus

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Abstract

The interference of eight components in the yield of sporulation and thermal resistance to moist heat (121°C) of Bacillus stearothermophilus spores suspended in 0.02 M calcium acetate solution and inoculated on paper strips previously treated with calcium acetate/calcium hydroxide was studied. The spore yield of 1.0×108/mL was developed at 62°C in 17 media containing different concentrations of d-glucose, sodium chloride, l-glutamic acid, yeast extract, peptone, manganese sulfate, potassium phosphate, and ammonium phosphate. The combined effects of yeast extract, peptone, and glucose contributed positively to the spore yield and to the stability of the thermal resistance of both spores in suspension and on strips.

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Authors and Affiliations

  1. Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of São Paulo, Rua Antonio de Macedo Soares, 452 04607-000, São Paulo/SP, Brazil

    Thereza Christina Vessoni Penna, Irene A. Machoshvili & Marina Ishii

Authors
  1. Thereza Christina Vessoni Penna
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  2. Irene A. Machoshvili
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  3. Marina Ishii
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Correspondence to Thereza Christina Vessoni Penna.

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Vessoni Penna, T.C., Machoshvili, I.A. & Ishii, M. Effect of media on spore yield and thermal resistance of Bacillus stearothermophilus . Appl Biochem Biotechnol 106, 287–294 (2003). https://doi.org/10.1385/ABAB:106:1-3:287

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  • DOI: https://doi.org/10.1385/ABAB:106:1-3:287

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