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Modified enzymes for reactions in organic solvents

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Abstract

Recent studies on biocatalysis in water—organic solvent biphasic systems have shown that many enzymes retain their catalytic activities in the presence of high concentrations of organic solvents. However, not all enzymes are organic solvent tolerant, and most have limited and selective tolerance to particular organic solvents. Protein modification or protein tailoring is an approach to alter the characteristics of enzymes, including solubility in organic solvents. Particular amino acids may play pivotal roles in the catalytic ability of the protein. Attaching soluble modifiers to the protein molecule may alter its conformation and the overall polarity of the molecule. Enzymes, in particular lipases, have been chemically modified by attachment of aldehydes, polyethylene glycols, and imidoesters. These modifications alter the hydrophobicity and conformation of the enzymes, resulting in changes in the microenvironment of the enzymes. By these modifications, newly acquired properties such as enhancement of activity and stability and changes in specificity and solubility in organic solvents are obtained. Modified lipases were found to be more active and stable in organic solvents. The optimum water activity (a w ) for reaction was also shifted by using modified enzymes. Changes in enantioselective behavior were also observed.

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References

  1. Klibanov, A. M. (1989), Trends Biotechnol. 14, 141–144.

    CAS  Google Scholar 

  2. Litjens, M. J. J., Sha, M., Straathof, A. J. J., Jongejan, J. A., and Heijnen, J. J. (1999), Biotechnol. Bioeng. 65, 347–356.

    Article  PubMed  CAS  Google Scholar 

  3. Tsuzuki, W., Kitamura, Y., Suzuki, T., and Kobayashi, S. (1999), Biotechnol. Bioeng. 64, 267–271.

    Article  PubMed  CAS  Google Scholar 

  4. Quiros, M., Parker, M. C., and Turner, N. J. (2001), J. Org. Chem. 66, 5074–5079.

    Article  PubMed  CAS  Google Scholar 

  5. Kwon, D. Y., Hong, Y. J., and Yoon, S. H. (2000), J. Agric. Food Chem. 48, 524–530.

    Article  PubMed  CAS  Google Scholar 

  6. Basri, M., Salleh, A. B., Ampon, K., Yunus, W. M. Z., and Razak, C. N. A. (1991), Biocatalysis 4, 313–317.

    CAS  Google Scholar 

  7. Ampon, K. Salleh, A. B., Salam, F., Yunus, W. M. Z., Razak, C. N. A., and Basri, M. (1991), Enzyme Microb. Technol. 13, 597–601.

    Article  CAS  Google Scholar 

  8. Basri, M., Ampon, K., Yunus, W. M. Z., Razak, C. N. A., and Salleh, A. B. (1992), J. Am. Oil Chem. Soc. 69(6), 579–583.

    CAS  Google Scholar 

  9. Takahashi, K., Saito, Y., and Inada, Y. (1988), J. Am. Oil Chem. Soc. 65, 911–916.

    Article  CAS  Google Scholar 

  10. Reslow, M., Adlercreutz, P., and Mattiasson, B. (1988), Eur. J. Biochem. 177, 313–318.

    Article  PubMed  CAS  Google Scholar 

  11. Zaks, A. and Klibanov, A. M. (1984), Science 224, 1249–1251.

    Article  PubMed  CAS  ADS  Google Scholar 

  12. Brink, L. E. S., Tamper, J., Luyben, J. C. A. M., and Van Piet, K. (1988), Enzyme Microb. Technol. 10, 736–743.

    Article  CAS  Google Scholar 

  13. Yang, Z. and Russel, A. J. (1996), in Enzymatic Reactions in Organic Media, Koskinen, A. M. P. and Klibanov, A. M., eds., Blackie Academic and Professional, Glasgow, UK.

    Google Scholar 

  14. Burke, P., Griffin, R. G., and Klibanov, A. M. (1993), Biotechnol. Bioeng. 42, 87–94.

    Article  CAS  Google Scholar 

  15. Ryu, K. and Dordick, J. S. (1989), J. Am. Chem. Soc. 111, 8026–8027.

    Article  CAS  Google Scholar 

  16. Laane, C. S., Boeren, S., Vos, K., and Veeger, C. (1987), Biotechnol. Bioeng. 30, 81–87.

    Article  CAS  Google Scholar 

  17. Bellota, J. C., Choisnarda, L., Castilloa, E., and Marty, A. (2001), Enzyme Microb. Technol. 28, 362–369.

    Article  Google Scholar 

  18. Means, G. E. and Feeney, R. G. (1968), Biochemistry 7, 1366–1371.

    Article  Google Scholar 

  19. Basri, M., Ampon, K., Yunus, W. M. Z., Razak, C. N. A., and Salleh, A. B (1995), J. Am. Oil Chem. Soc. 72(4), 407–411.

    CAS  Google Scholar 

  20. Bradford, M. M. (1976), Anal. Biochem. 72, 581–590.

    Article  Google Scholar 

  21. Fields, R. (1972), Biochemistry J. 124, 581–590.

    Google Scholar 

  22. Jasmani, H., Basri, M., Razak, C. N. A., Salleh, A. B., and Ahmad, F. B. H. (2001), Mal. J. Anal. Sci. 7, 209–212.

    Google Scholar 

  23. Dordick, J. S. (1989), Enzyme Microb. Technol. 11, 194–211.

    Article  CAS  Google Scholar 

  24. Ampon, K., Salleh, A. B., Teoh, A., Yunus, W. M. Z., Razak, C. N. A., and Basri, M. (1991), Biotechnol. Lett. 13(1), 25–30.

    Article  CAS  Google Scholar 

  25. Ampon, K., Salleh, A. B., Basri, M., Yunus, W. M. Z., Razak, C. N. A., and Whitaker, J. R. (1993), J. Biosci. 4(2), 124–160.

    Google Scholar 

  26. Salleh, A. B., Ampon, K., Salam, F., Yunus, W. M. Z., Razak, C. N. A., and Basri, M. (1990), Ann. NY Acad. Sci. 613, 521–522.

    Article  CAS  Google Scholar 

  27. Longo, M. A. and Combes, D. (1999), J. Chem. Tech. Biotechnol. 74, 25–32.

    Article  CAS  ADS  Google Scholar 

  28. Hernaiz, M. J., Sanches-Montero, J. M., and Sinisterra, J. V. (1999), Biotechnol. Bioeng. 55(2), 252–260.

    Article  Google Scholar 

  29. Zaks, A. and Klibanov, A. M. (1988), J. Biol. Chem. 263, 8017–8021.

    PubMed  CAS  Google Scholar 

  30. Halling, P. J. (1989), Trends Biotechnol. 7, 50–51.

    Article  CAS  Google Scholar 

  31. Valivety, R. H., Halling, P. J., and Macrae, A. R. (1992), Biochim. Biophys. Acta 1122, 143–146.

    PubMed  CAS  Google Scholar 

  32. Basri, M., Ampon, K., Yunus, W. M. Z., Razak C. N. A., and Salleh A. B. (1994), J. Chem. Tech. Biotech. 59, 37–44.

    Article  CAS  Google Scholar 

  33. Basri, M., Th’ng, B. L., Razak C. N. A., and Salleh A. B. (1998), Ann. NY Acad. Sci. 864, 192–198.

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. M. Taib

    Present address: Fakulti Sains & Teknologi, Universiti Kolej Terengganu, 21030, Kuala Terengganu, Malaysia

  2. H. Jasmani

    Present address: Jabatan Kimia, Fakulti Sains, Universiti Teknologi Mara, 40450, Shah Alam, Malaysia

Authors and Affiliations

  1. Center for Research in Enzyme & Microbial Technology (CREAM), Fakulti Sains & Pengajian Alam Sekitar, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia

    A. B. Salleh, M. Basri, M. Taib, H. Jasmani, R. N. Z. A. Rahman, M. B. A. Rahman & C. N. A. Razak

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  1. A. B. Salleh
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  2. M. Basri
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Correspondence to A. B. Salleh.

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Salleh, A.B., Basri, M., Taib, M. et al. Modified enzymes for reactions in organic solvents. Appl Biochem Biotechnol 102, 349–357 (2002). https://doi.org/10.1385/ABAB:102-103:1-6:349

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  • DOI: https://doi.org/10.1385/ABAB:102-103:1-6:349

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