The actin-binding domain of spinophilin is necessary and sufficient for targeting to dendritic spines
- 184 Downloads
Spinophilin is enriched in dendritic spines, small protrusions of the postsynaptic membrane along the length of the dendrite that contain the majority of excitatory synapses. Spinophilin binds to protein phosphatase 1 with high affinity and targets it to dendritic spines, therefore placing it in proximity to regulate glutamate receptor activity. Spinophilin also binds to and bundles f-actin, the main cytoskeletal constituent of dendritic spines, and may therefore serve to regulate the structure of the synapse. In this study, we sought to determine the structural basis for the targeting of spinophilin to dendritic spines. Our results show that the actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines.
Index EntriesSpinophilin Neurabin II dendritic spines actin postsynaptic density
Unable to display preview. Download preview PDF.
- Barnes A., Smith F., and Milgram S. (2001) Identification of a novel actin-binding site within spinophilin/neurabinII. Abstracts of the 41st annual Meeting of the American Society for Cell Biology.Google Scholar
- Goslin K. and Banker G. (1991) Rat hippocampal neurons in low density culture, in Culturing Nerve Cells. MIT, Cambridge, MA, pp. 339–370.Google Scholar
- Gray E. G. (1959) Axosomatic and axodendritic synapses of the cerebral cortex: an electron microscopic study. J. Anat. 83, 420–433.Google Scholar
- Hsieh-Wilson L. C., Benfenati F., Snyder G. L., Allen P. B., Nairn A. C., and Greengard P. (2002) Phosphorylation of spinophilin modulates its interaction with actin filaments. J. Biol. Chem. (Submitted.)Google Scholar
- Smith F. D., Oxford G. S., and Milgram S. L. (1999) Association of the D2 dopamine receptor third cytoplasmic loop with spinophilin, a protein phosphatase-1-interacting protein. J. Biol. Chem. 274, 19,894–19,900.Google Scholar
- Vivo M., Calogero R. A., Sansone F., Calabro V., Parisi T., Borrelli L., et al. (2001) The human tumor suppressor arf interacts with spinophilin/neurabin II, a type 1 protein-phosphatase-binding protein. J. Biol. Chem. 276, 14,161–14,169.Google Scholar