NeuroMolecular Medicine

, Volume 2, Issue 1, pp 61–69

The actin-binding domain of spinophilin is necessary and sufficient for targeting to dendritic spines

  • Stacie D. Grossman
  • Linda C. Hsieh-Wilson
  • Patrick B. Allen
  • Angus C. Nairn
  • Paul Greengard
Original Research

Abstract

Spinophilin is enriched in dendritic spines, small protrusions of the postsynaptic membrane along the length of the dendrite that contain the majority of excitatory synapses. Spinophilin binds to protein phosphatase 1 with high affinity and targets it to dendritic spines, therefore placing it in proximity to regulate glutamate receptor activity. Spinophilin also binds to and bundles f-actin, the main cytoskeletal constituent of dendritic spines, and may therefore serve to regulate the structure of the synapse. In this study, we sought to determine the structural basis for the targeting of spinophilin to dendritic spines. Our results show that the actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines.

Index Entries

Spinophilin Neurabin II dendritic spines actin postsynaptic density 

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Copyright information

© Humana Press Inc 2002

Authors and Affiliations

  • Stacie D. Grossman
    • 1
  • Linda C. Hsieh-Wilson
    • 1
    • 2
  • Patrick B. Allen
    • 1
  • Angus C. Nairn
    • 1
    • 3
  • Paul Greengard
    • 1
  1. 1.Laboratory of Molecular and Cellular NeuroscienceThe Rockefeller UniversityNew York
  2. 2.Division of Chemistry and Chemical EngineeringCalifornia Institute of TechnologyPasadena
  3. 3.Department of PsychiatryYale University School of MedicineNew Haven

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