The informatics of a C57BL/6J mouse brain atlas
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The Mouse Atlas Project (MAP) aims to produce a framework for organizing and analyzing the large volumes of neuroscientific data produced by the proliferation of genetically modified animals. Atlases provide an invaluable aid in understanding the impact of genetic manipulation by providing a standard for comparison. We use a digital atlas as the hub of an informatics network, correlating imaging data, such as structural imaging and histology, with text-based data, such as nomenclature, connections, and references. We generated brain volumes using magnetic resonance microscopy (MRM), classical histology, and immunohistochemistry, and registered them into a common and defined coordinate system. Specially designed viewers were developed in order to visualize multiple datasets simultaneously and to coordinate between textual and image data. Researchers can navigate through the brain interchangeably, in either a text-based or image-based representation that automatically updates information as they move. The atlas also allows the independent entry of other types of data, the facile retrieval of information, and the straight-forward display of images. In conjunction with centralized servers, image and text data can be kept current and can decrease the burden on individual researchers’ computers. A comprehensive framework that encompasses many forms of information in the context of anatomic imaging holds tremendous promise for producing new insights. The atlas and associated tools can be found at http://www.loni.ucla.edu/MAP.
Index EntriesAnatomy atlas brain C57BL/6 gene expression mouse MRM
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- Bota, M. and Arbib M. A. (2002) The Neurohomology database: An online-KMS for handling and evaluation of neurobiological information, in A Practical Guide to Neuroscience Databases and Associated Tools. (Kotter, R., ed.) Kluwer Academic Publishers, Boston, MA. pp. 203–220.Google Scholar
- Franklin, K. B. J. and Paxinos, G. (1997) The Mouse Brain in Stereotaxic Coordinates, Academic Press, San Diego.Google Scholar
- Gallyas, F. (1979) Silver staining of myelin by means of physical development. Neurol. Res. 1, 203–209.Google Scholar
- Hof, P. R. and Young, W. G. (2000) Comparative Cytoarchitectonic Atlas of the C57BL 6 and 129 Sv Mouse Brains, Elsevier, Amsterdam.Google Scholar
- Paxinos, G. and Watson, C. (1998) The Rat Brain in Stereotaxic Coordinates, 4th ed., Academic Press, San Diego.Google Scholar
- Paxinos, G. and Franklin, K. B. J. (2001) The Mouse Brain in Stereotaxic Coordinates, 2nd ed., Academic Press, San Diego.Google Scholar
- Rosen, G. D., Williams, A. G., Capra, J. A., et al. (2000) The Mouse Brain Library @www.mbl.org.Google Scholar
- Simmons, D. M. and Swanson, L. W. (1993) The Nissl Stain, in Neuroscience Protocols, Wouterlood, F. G., ed., Elsevier, Amsterdam, pp. 93-050-12-1–93-050-12-7.Google Scholar
- Swanson, L. W. (1998) Brain Maps: Structure of the Rat Brain, 2nd ed., Elsevier, Amsterdam.Google Scholar
- Toga, A. W. and Thompson, P. M. (1998) Multimodal Brain Atlases, in Medical Image Databases. Kluwer Academic Press, Dordrecht, The Netherlands, pp. 53–88.Google Scholar