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NanoBiotechnology

, Volume 1, Issue 1, pp 83–92 | Cite as

Carbon-nanotube-modified electrodes for the direct bioelectrochemistry of pseudoazurin

  • Anthony Guiseppi-Elie
  • Sean Brahim
  • Gary Wnek
  • Ray Baughman
Original Article

Abstract

The bioelectrochemistry of the blue copper protein, pseudoazurin, at glassy carbon and platinum electrodes that were modified with single-wall carbon nanotubes (SWNTs) was investigated by multiple scan rate cyclic voltammetry. The protein showed reversible electrochemical behavior at both bare glassy carbon electrodes (GCEs) and SWNT-modified GCEs (SWNT|GCEs); however, direct electrochemistry was not observed at any of the platinum electrodes. The effect of the carbon nanotubes at the GCE was to amplify the current response 1000-fold (nA at bare GCE to µA at SWNT|GCE), increase the apparent diffusion coefficient D app of the solution-borne protein by three orders of magnitude, from 1.35 × 10−11 at bare GCE to 7.06 × 10−8 cm2 s-1 at SWNT|GCE, and increase the heterogeneous electron transfer rate constant k s threefold, from 1.7 × 10−2 cm s−1 at bare GCE to 5.3 × 10−2 cm s−1 at SWNT|GCE. Pseudoazurin was also found to spontaneously adsorb onto the nanotube-modified GCE surface. Well-resolved voltammograms indicating quasi-reversible faradaic responses were obtained for the adsorbed protein in phosphate buffer, with I pc and I pa values now greater than corresponding values for solution-borne pseudoazurin at SWNT|GCEs and with significantly reduced ΔE p values. The largest electron transfer rate constant of 1.7 × 10−1 cm s−1 was achieved with adsorbed pseudoazurin at the SWNT|GCE surface in deaerated buffer solution consistent with its presumed role in anaerobic respiration of some bacteria.

Key Words

Carbon nanotubes nanoelectrodes metalloproteins 

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Copyright information

© Humana Press Inc 2005

Authors and Affiliations

  • Anthony Guiseppi-Elie
    • 1
    • 3
  • Sean Brahim
    • 1
  • Gary Wnek
    • 1
    • 3
  • Ray Baughman
    • 2
  1. 1.Center for Bioelectronics, Biosensors, and Biochips (C3B)Virginia Commonwealth UniversityRichmondUSA
  2. 2.Department of Chemistry and Nanotechnology CenterUniversity of Texas at DallasRichardsonUSA
  3. 3.Department of Chemical Engineering, Center for Bioelectronics, Biosensors, and Biochips (C3B)Virginia Commonwealth UniversityRichmondUSA

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